Downloaded from www.microbiologyresearch.org by IP: 54.198.95.179 On: Fri, 09 Sep 2016 06:59:52 Promicromonospora umidemergens sp. nov., isolated from moisture from indoor wall material K. Martin, 1 J. Scha ¨ fer 2 and P. Ka ¨ mpfer 2 Correspondence P. Ka ¨ mpfer peter.kaempfer@umwelt.uni- giessen.de 1 Institut Leibniz-Institut fu ¨ r Naturstoff-Forschung und Infektionsbiologie eV, Hans-Kno ¨ ll-Institut, D-07745 Jena, Germany 2 Institut fu ¨ r Angewandte Mikrobiologie, Justus-Liebig-Universita ¨ t Giessen, D-35392 Giessen, Germany A Gram-positive, yellow-pigmented, branched-hyphae-forming micro-organism, strain 09-Be- 007 T , was isolated from the wall of an indoor environment. Based on 16S rRNA gene sequence similarity studies, strain 09-Be-007 T belonged to the genus Promicromonospora. The novel isolate showed sequence similarities of 98.8 % to Promicromonospora aerolata V54A T , 98.9 % to Promicromonospora vindobonensis V45 T , 98.1 % to Promicromonospora sukumoe DSM 44121 T , 98.2 % to Promicromonospora kroppenstedtii RS16 T , 96.7 % to Promicromonospora flava CC 0387 T and 97.8 % to Promicromonospora citrea DSM 43110 T , the type strain of the type species of this genus. Cell wall sugars of strain 09-Be-007 T were galactose, rhamnose and glucose. The diagnostic diamino acid of the cell-wall peptidoglycan was lysine. The major menaquinones detected were MK-9(H 4 ) and MK-9(H 6 ). Major polar lipids were diphosphatidylglycerol, phosphatidylglycerol and phosphatidylinositol. Major fatty acids were iso- C 15 : 0 and anteiso-C 15 : 0 ; moderate amounts of anteiso-C 17 : 0 , iso-C 16 : 0 and iso-C 15 : 1 were also found. All these data supported the affiliation of strain 09-Be-007 T to the genus Promicromonospora. DNA–DNA hybridization values and physiological and biochemical data enabled strain 09-Be-007 T to be differentiated genotypically and phenotypically from the six recognized species of the genus Promicromonospora. For these reasons, strain 09-Be-007 T represents a novel species, for which the name Promicromonospora umidemergens sp. nov. is proposed, with 09-Be-007 T (5DSM 22081 T 5CCM 7634 T ) as the type strain. The genus Promicromonospora currently harbours six species: Promicromonospora citrea (Krasil’nikov et al., 1961), Promicromonospora sukumoe (Takahashi et al., 1987), Promicromonospora aerolata and Promicro- monospora vindobonensis (Busse et al., 2003), Promicro- monospora kroppenstedtii (Alonso-Vega et al., 2008), and Promicromonospora flava (Jiang et al., 2009). Two species originally described as members of the genus Promicro- monospora have been transferred to other genera: Promicromonospora enterophila (Ja ¨ger et al., 1983) was transferred to the genus Oerskovia as Oerskovia enterophila (Stackebrandt et al., 2002), and Promicromonospora pachnodae (Cazemier et al., 2003) was reclassified as Xylanimicrobium pachnodae (Stackebrandt & Schumann, 2004). P. citrea, P. sukumoe and P. kroppenstedtii were isolated from soil samples, whereas P. aerolata and P. vindobonensis were isolated from the air of the medieval Virgilkapelle in Vienna. P. flava was isolated from sediment from the Baltic Sea in Germany. Strain 09-Be-007 T was enriched and recovered from a wall that was colonized with moulds. After extraction of 1 g sample material by shaking for 15 min in 10 ml 0.9 % NaCl solution containing 0.01 % (v/v) Tween 80, aliquots of this suspension were spread on agar plates containing organic medium M79 agar (Prauser & Falta, 1968) and incubated for 2 weeks at 28 uC. The new strain was maintained on M79 agar and preserved at 280 u C by mixing well-grown M79 broth cultures in a 1 : 1 ratio with glycerol preservation medium (Salser, 1978) containing K 2 HPO 4 (1.26 %), KH 2 PO 4 (0.36 %), MgSO 4 . 7H 2 O (0.01 %), sodium citrate (0.09 %), (NH 4 ) 2 SO 4 (0.18 %) and glycerol (8.80 %). Stock cultures of the isolate in liquid M79 supplemented with 5 % DMSO were also maintained in the vapour phase of liquid nitrogen. Morphological properties, Gram-staining and cell mor- phology were observed microscopically as described by Ka ¨mpfer & Kroppenstedt (2004). Strain 09-Be-007 T formed yellow-pigmented colonies with characteristic wrinkly and shining surfaces. Branching septate hyphae, which grew on the agar surface and penetrated into the The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain 09-Be-007 T is FN293378. International Journal of Systematic and Evolutionary Microbiology (2010), 60, 537–541 DOI 10.1099/ijs.0.014142-0 014142 G 2010 IUMS Printed in Great Britain 537