Poster Presentations / Pregnancy Hypertension 1, Supplement 1 (2010) S43–S76 S55 and 0.4g/24h for PE and 2.9mg/dl and 4.8g/24h for PG. Podocyte cells were observed in all three groups, but there were only significant numbers in PG and PE. In PE group it was found almost three times the quantity detected in PG. Conclusions: Urinary podocyte excretion occurs in PE and PG besides other renal abnormalities; furthermore, it may contribute to the development of glomerulosclerosis. The detection of podocyturia in PE could be useful for preventing, diagnosing and following up the glomerular injury and it may also be correlated to its severity or activity, although additional studies are necessary to confirm these points. Apoio FAPESP 08/56338-1 P47 Importance of renal function evaluation during pregnancy performed by obstetricians/gynecologists and residents Thaís A. Facca 1 , Gianna M. Kirsztajn 1 , Frederico R. Ghersel 2 , Henry Korkes 3 , Nelson Sass 1 . 1 Federal University of São Paulo, São Paulo, Brazil; 2 ABC Medical School, Brazil; 3 Maternidade Escola de Vila Nova Cachoeirinha, Brazil Background: In normal pregnancy there is an increase of 50% in glomerular filtration rate and small elevations of serum creatinine level (CREA) may characterize a significant decline in renal function, especially if associated with hypertension, when there may be a reduction of the glomerular filtration surface. Renal failure (RF) is an important disease with insidious onset, difficult to diagnose and, in pregnant women, its mortality rate can reach 20% and compromise perinatal outcomes. Aims: To assess the knowledge on renal function evaluation and kind of investigation performed during pregnancy by obstetricians/gynecologists (OB/GYN) and residents in public hospitals. Methods: Physicians of the OB/GYN department of five public hospitals in Sao Paulo-Brazil answered a questionnaire with ten multiple choice questions about renal evaluation in pregnancy, such as exams in prena- tal, diagnostic tests for preeclampsia (PE) and reference values (RV) for pregnant women. Results: Among 65 physicians, 60% (39/65) were OB/GYN attendants and 40% (26/65) OB/GYN residents. The mean age was 33.2 years and the mean of years of graduation was 9. In the first prenatal appointment: 87% (57/65) answered that usually order urinalysis test; and for hypertensive women, 70% (46/65) add urea and CREA. About RV of proteinuria for diagnosing PE: only 29% (19/65) answered ≥1+ for dipstix; only 20% (13/65) marked 1g for isolated urine sample, and 81% (53/65) 300mg for 24-hour proteinuria. About RV for elevated CREA in pregnancy, only 21% (14/65) answered ≥0.8mg/dl; for CREA corresponding to RF in pregnancy only 26% (17/65) marked ≥1.2mg/dl and about the value of urinary output corresponding to oliguria only 53% (35/65) checked ≤30ml/h. Conclusions: It was not observed accordance among physicians about fun- damental concepts related to diagnosis of renal involvement in pregnancy. OB/GYN and nephrology professionals have to highlight these relevant aspects during graduation and medical residence. P48 Technique of B-Lynch in the control of postpartum hemorrhage in hypertensive pregnant women: report of three cases Nelson Sass, Elizabet K. Watanabe, Henry Korkes, Sylvia M. Almeida, Gilberto Nagahama. Maternidade Escola de Vila Nova Cachoeirinha, Brazil Background: Postpartum hemorrhage (PPH) is a leading cause of maternal mortality and uterine atony is a common cause. There are many alternatives for treatment such as the use of oxytocin, prostaglandins, misoprostol and surgical techniques such as arterial ligation, uterine compression sutures, angiographic embolization and hysterectomy. We report three cases using the technique of B-Lynch in hypertensive pregnant women. Reports: Case 1: 23 years, in her second pregnancy one previous vaginal delivery and gestational age (GA) = 37 weeks, hospitalized for severe pre-eclampsia. After maternal stabilization with magnesium sulfate and hydralazine, cesarean was performed followed massive hemorrhage due to uterine atony. After initial attempt with oxytocin and misoprostol without success, was performed B-Lynch technique successfully. The pa- tient required blood transfusion and was discharged without further complications. Case 2: 27 years, first pregnancy, GA = 36 weeks admitted in imminent eclampsia. She was treated with magnesium sulfate and hydralazine and underwent cesarean section after maternal stabilization. It was noted uter- ine inversion followed by atony and, after initial procedures, was carried out B-Lynch suture successfully. Evolved with anemia and wound infection and was discharged 13 days after delivery. Case 3: 22 year old and GA = 36 weeks. Started induction of labor because of pre-eclampsia and indicated cesarean section for fetal distress, followed for uterine atony. After medical proceeds, it was performed B-Lynch suture successfully and was discharged uneventfully on day 3 postpartum. Conclusion: The B-Lynch haemostatic suture can be an alternative for the control of PPH. This suture has proven easy to understand and im- plement, preserving fertility, which makes it an effective alternative for the treatment of PPH in these patients, reducing the need to perform hysterectomies, even in hypertensive patients, especially in young women with low parity. P49 Serum lipid levels in pregnancies complicated by preeclampsia Valmir Jose de Lima 1 , Gustavo E. Ruschi 2 , Fabiana Lima Marques 3 , Nelson Saas 1 . 1 Obstetrics Department, São Paulo Federal University, São Paulo, Brazil; 2 Santa Casa de Vitória Medical School; 3 Vila Velha University, Brazil Objective: Analyze the lipid profile (fractions and total serum cholesterol and triglyceride levels) of patients with pre-eclampsia and investigate possible correlations between the levels these compounds and the degree of 24 h proteinuria. Methods: Case-control study involving 42 patients with pre-eclampsia and 35 healthy pregnant women (control group) managed at the Dório Silva Hospital, in the city of Vitória, Espirito Santo, Brazil, between June 2005 and January 2008. After a 12 hour fast, blood was collected to determine the plasmatic concentration of triglycerides, total cholesterol and fractions (LDL-C, HDL-C and VLDL-C). The results of the lipid profile were classified according to the criteria proposed by the Brazilian Society of Cardiology (SBC). The degree of proteinuria was evaluated in the volume of urine collected over a 24-hour period. Cases and controls were matched for maternal age, gestational week and body mass index. Results: The total serum cholesterol and triglyceride levels of pre-eclamptic patients were more frequently above the normal range established by the SBC criteria. The mean VLDL-C and triglyceride levels of women with pre-eclampsia differed significantly from control women. In pre-eclamptic patients there was a positive association between increasing proteinuria and higher concentrations of VLDL-C and triglycerides. Conclusion: The serum cholesterol and triglyceride levels of pre-eclamptic women are more frequently in the range considered as abnormal, these women have higher mean concentrations of triglyceride and VLDL choles- terol than normal pregnant women. Similarly, these levels seem to be pos- itively correlated with increasing proteinuria, suggesting that worsening of the pathophysiological process is accompanied by metabolic abnormalities. Our findings seem to indicate that patients with pre-eclampsia should receive a special follow-up during the course of their lives since they may be at high risk for cardiovascular events. P50 Comparative assessment of levels zinc in preeclampsia and gestational hypertension Pedro Paulo Corrales Faria 1 , Ana Carolina Palei 2 , Valéria Cristina Sandrim 1 , Geraldo Duarte 1 , José Eduardo Tanus-Santos 1 , Ricardo Cavalli 1 . 1 Faculty of Medicine of Ribeirão Preto - USP, Brazil; 2 Faculty of Pharmaceutical Sciences of Ribeirão Preto - USP, Brazil Background: Several reports had suggested that zinc deficiency may be associated with increased incidence of preeclampsia. Objectives: To compare the circulating levels of zinc in preeclampsia and gestational hypertension with those found in normotensive pregnancies. Design and methods: We studied 150 pregnant women (51 healthy pregnant women with uncomplicated pregnancies, 61 with gestational hypertension, and 38 with preeclampsia) and 35 healthy non pregnant women. Zinc levels were measured in plasma samples by simple method based on ICP-MS. Results: The serum levels of zinc in non-pregnant group were 623.7±19.21 μg/l and 492.6±13.79 μg/l; 537.1±14.34 μg/l and 514.2±16.09 μg/l in healthy pregnant, gestational hypertension and preeclampsia respectively. No differences were observed in the levels of the zinc among groups (all p>0.05). Conclusions: These results indicate that serum levels of zinc during preg- nancy might not be possible contributors in etiology of pre-eclampsia, and