Spectrochimica Acta Part B 57 (2002) 581–590 0584-8547/02/$ - see front matter  2002 Elsevier Science B.V. All rights reserved. PII: S0584-8547 Ž 01 . 00386-X Fractionation and speciation of Cu, Zn and Fe in wine samples by atomic absorption spectrometry  Irina Karadjova , Belgin Izgi , Seref Gucer * a b b, Faculty of Chemistry, University of Sofia, 1164 Sofia, Bulgaria a Department of Chemistry, Faculty of Science & Arts, Uludag University, 16059 Bursa, Turkey b Received 14 August 2001; accepted 20 November 2001 Abstract A scheme is presented for fractionation of wine components and Fe, Cu and Zn determination in different fractions. The charge of the metal species was established using cation and anion exchange separation based on solid phase extraction. The resin XAD-8 was used for the separation of wine polyphenols in complexes with wine proteins and polysaccharides. Dowex ion exchange resins were used for the separation of cationic and anionic species of Fe, Cu and Zn. Flame atomic absorption spectrometry and electrothermal atomic absorption spectrometry were used off-line for the quantitative determination of metals in the different fractions obtained.  2002 Elsevier Science B.V. All rights reserved. Keywords: Fractionation; Speciation; Fe; Cu; Zn; Atomic absorption spectrometry; Wine 1. Introduction The speciation of metals with biological ligands is a subject of increasing interest since complexa- tion may reduce their toxicity and their bioavaila- bility. Speciation of Al and Mn in tea infusions was reported in our earlier studies w1–3x. Wine is a widely consumed beverage with thousands of years of tradition. However, there is  This paper was presented at Colloquium Spectroscopicum Internationale XXXII, held in Pretoria, South Africa, 8–13 July 2001 and is published in the special issue of Spectrochim- ica Acta Part B, dedicated to that conference. *Corresponding author. Tel.: q90-224-442-9256; fax: q90- 224-442-8136. E-mail address: sgucer@uludag.edu.tr (S. Gucer). a limited number of studies concerning the speci- ation of metals in wines. Differential pulse anodic stripping voltammetry and ion-selective potentiom- etry have been used for the simultaneous deter- mination of free metal ions and labile and tightly bound metal complexes for Cu and Pb w4–7x. Several chromatographic studies also discussed ion complexation in wine samples w8–10x. Wine is a very complex matrix and the accurate determina- tion of particular chemical forms of metals in wine is a real analytical challenge. Size fractionation of metals in wine using ultrafiltration w11x, and spe- ciation of Fe w12x, Cd, Cu and Pb using different solid sorbents w13x were reported. In the present paper an attempt is given in for wine sample fractionation and Fe, Cu and Zn determination in