Colloids and Surfaces A: Physicochemical and Engineering Aspects 170 (2000) 107 – 112 Controversy about -tricalcium phosphate M. Tonkovic ´ *, M. Sikiric ´ 1 , V. Babic ´-Ivanc ˇic ´ Department of Chemistry, Rudjer Bos ˇkoic ´ Institute, Bijenic ˇka 54, 10000 Zagreb, Croatia Received 4 June 1999; accepted 28 January 2000 Abstract The calcium phosphate which corresponds to the formula Ca 3 (PO) 4 · n H 2 O (2 n 3) was isolated from solutions with Ca/P molar ratio 0.2 and pH 7. The compound was characterised by chemical and thermogravimetric analyses, Fourier-transform infrared (FTIR) spectroscopy and X-ray diffraction. The FTIR spectra were compared with spectra of -tricalcium phosphate (-TCP) in the atlases for analysis of urinary calculi and other literature data. © 2000 Elsevier Science B.V. All rights reserved. Keywords: Tricalcium phosphate hydrate; -Tricalcium phosphate; Urinary calculi; Fourier-transform infrared; X-ray diffraction www.elsevier.nl/locate/colsurfa 1. Introduction Accurate knowledge of the composition of hu- man urinary calculi is essential to the study of their formation and the choice of medical treat- ments. The determination of urinary calculi com- position is usually made by the infrared (IR) spectroscopy or X-ray diffraction (XRD), which are considered the most rapid and reliable analyt- ical methods for this purpose. Various calcium phosphates were identified as either major or minor components of human uri- nary calculi. Because of their physiological rele- vance, the formation and transformation behaviour of these compounds has been exten- sively studied. It has been shown that the types of calcium phosphate precipitate are influenced by the various factors such as pH, temperature, ionic concentration, Ca/P molar ratio, rate of stirring and digestion time. Under conditions of high su- persaturation the initial Ca/P molar ratio of the solution has no significant effect on the type Ca-phosphate formed, whereas under conditions of low supersaturation the initial Ca/P molar ratio of the solution exerts an influence. Among all calcium phosphates, the carbonate apatite Ca 10 (PO 4 )(CO 3 OH) 6 (OH) 2 and calcium hydrogen phosphate dihydrate CaHPO 4 · 2H 2 O (DCPD, brushite) are considered the frequent urinary cal- culi substances; -tricalcium phosphate Ca 3 (PO 4 ) 2 (-TCP, whitlockite) and amorphous calcium phosphate Ca 3 (PO 4 ) 2 as rare urinary calculi substances and calcium hydrogen phosphate CaHPO 4 (monetite), octa calcium phosphate * Corresponding author. Tel.: +385-1-4561007; fax: +385- 1-4680245. E-mail address: tonkovic@rudjer.irb.hr (M. Tonkovic ´) 1 Present address: Faculty of Agriculture, University of Za- greb, Zagreb, Croatia. 0927-7757/00/$ - see front matter © 2000 Elsevier Science B.V. All rights reserved. PII:S0927-7757(00)00476-3