Ab Initio Calculation of Isotopic Fractionation in B(OH) 3 (aq) and BOH 4 - (aq) James R. Rustad* and Eric J. Bylaska Department of Geology, UniVersity of California, DaVis, One Shields AVenue, DaVis, California 95616, and Pacific Northwest National Laboratory, MS K9-96, Richland, Washington 99352 Received November 21, 2006; E-mail: jrrustad@ucdavis.edu Computational methods are becoming increasingly central to understanding the fractionation of stable isotopes in geochemical systems. A prominent application is the fractionation of 11 B and 10 B between boric acid B(OH) 3 and borate ion B(OH) 4 - in seawater. Boron isotopes are key indicators of the pH and CO 2 content of oceans in the geologic past. 1 In seawater solutions at 25 °C and [B] total ) 4.5 ppm, B(OH) 3 and B(OH) 4 - exist in equal concentra- tions at pH 8.8. Since the 11 B/ 10 B ratio differs between B(OH) 3 and B(OH) 4 - , the isotopic composition of each species is a function of pH over the range where both species coexist in appreciable concentrations. If it is assumed that only B(OH) 4 - is incorporated into minerals, then the pH of seawater in equilibrium with the min- erals at the time of deposition can be obtained from the 11 B/ 10 B ratio in the mineral phases. The calculation of pH requires know- ledge of the isotope fractionation factor R 34 . In the harmonic limit, R 34 ) B(OH)3 / B(OH)4 - ( 3 / 4 ) where is the reduced partition function ratio: where u (h,l)i ) pc2πω (h,l)i /kT, h and l refer to the heavy isotope and light isotopes, respectively, and the product runs over all frequencies. 2 Paleo-ocean pH estimates have until recently relied on semiem- pirical estimates of R 34 , 3 in part because of the difficulty of measurements at [B] total ) 4.5 ppm. Several problems have lately been identified in the original work, and improvements have been sought through electronic structure calculations. 4,5 Recent measure- ments of the isotope-induced shift in the pK a of B(OH) 3 have reported R 34 values close to 1.028, within 1 per mil of the Hartree- Fock calculations. 5c-e This level of agreement is surprising as the Hartree-Fock calculations involve extensive frequency scaling. The approach combines the effects of solvation, inadequacies in the wave function, and anharmonicity into a single factor, making systematic improvement difficult. Here, we take a new approach to estimating R 34 for aqueous species, using ab initio molecular dynamics (AIMD). 6 The AIMD approach is useful because the fractionating species are embedded in real solvent at configurations typical of 300 K. Moreover, the vibrational frequencies obtained through AIMD are not restricted to be harmonic. This study is the first time AIMD has been applied to the calculation of isotopic fractionation. First, we establish whether the AIMD calculations are capable of reproducing the observed frequencies for B(OH) 4 - (aq) and B(OH) 3 (aq). Second, we explore the utility of eq 1 in reproducing R 34 . Work on gas- phase systems, for example, indicates that it is better to use harmonic frequencies in eq 1 rather than anharmonic frequencies derived from experiment. 7 It is not clear whether this would remain true in aqueous systems. B(OH) 4 - and B(OH) 3 are inserted in a small periodic cell of water molecules, and forces are calculated using density functional theory. The forces are used to perform a molecular dynamics simulation. The classical vibrational density of states responsible for boron fractionation can then be obtained by Fourier transforma- tion of the velocity autocorrelation function for the boron atom. All calculations were carried out with NWChem, 8 employing a plane-wave basis (energy cutoff ) 90 au) with Troullier-Martins pseudopotentials 9 and the PBE96 exchange-correlation functional. 10 We used Car-Parrinello dynamics with the fictitious mass set at 100 au. Electronic and nuclear degrees of freedom were attached ² University of California, Davis. Pacific Northwest National Laboratory. ) ( Q h Q l 29 ) i u hi u li e -u hi /2 1 - e -u hi 1 - e -u li e -u li /2 (1) Figure 1. Vibrational density of states for the boron atom in (a) B(OH)3- (aq), (b) B(OH)4 - (aq), and (c) teepleite (Na2ClB(OH)4). Green: 11 B; Blue: 10 B. Multiple lines represent spectra calculated from replicas of the VAF within the standard error. Published on Web 02/01/2007 2222 9 J. AM. CHEM. SOC. 2007, 129, 2222-2223 10.1021/ja0683335 CCC: $37.00 © 2007 American Chemical Society