World Journal of Fish and Marine Sciences 8 (2): 74-84, 2016 ISSN 2078-4589 © IDOSI Publications, 2016 DOI: 10.5829/idosi.wjfms.2016.8.2.10328 Corresponding Author: Manal M. El-Naggar, Microbiology Laboratory, Marine Environmental Division, National Institute of Oceanography and Fisheries, Alexandria, Egypt. 74 Production of Anti-Inflammatory Pyrrol Compound from Marine Bacillus baekryungensis AMHSU Manal M. El-Naggar, Hanan M. Abd-Elnaby, Seham A. Abou-Shousha, 1 1 2 Usama M. Abdul-Raouf and Ahmed E. Abouelwafa 3 1 Microbiology Laboratory, Marine Environmental Division, 1 National Institute of Oceanography and Fisheries, Alexandria, Egypt Department of Immunology &Allergy, Medical research Institute, Alexandria University, Egypt 2 Department of Botany and Microbiology, Faculty of Science, Al-Azhar University, Assiut, Egypt 3 Abstract: Working on marine microbial resources may lead to the discovery of an alternative anti-inflammatory agent added to the commercial used ones. Marine bacterial isolates were screened for their ability to produce anti-inflammatory agent(s) using a preliminary screening test “inhibition of albumin denaturation assay” using the bovine serum albumin compared to diclofenac sodium (a standard anti-inflammatory drug). The obtained results indicated the isolation of a new marine bacterial strain from the western harbor of Alexandria, it identified as Bacillus baekryungensis AMHSU using the 16S rRNA gene sequence. It showed the most potent anti-inflammatory activity 46.78±2.81% compared to the standard diclofenac sodium 28.56±1.15%. The optimization process was performed using the Plackett –Burman experimental design and the propagation of the anti-inflammatory activity was carried out through the adsorption immobilization technique using the luffa pulp supporter. The optimized medium maximized the anti-inflammatory activity by 1.46 fold compared to the basal medium (62.32%). While the adsorbed cells was efficiently maximize the anti-inflammatory activity (95.36±0.64). The chemical characterization of the ethyl-acetate extract was performed using the IR, NMR and GC Ms spectrophotometry, these analysis indicated the most probable effective compound as anti-inflammatory agent according to the obtained data was pyrrolo [1, 2-a]pyrazine-1, 4-dione derivatives with a similarity of 91%. Key words: Anti-Inflammatory Pyrrol Bacillus Bovine serum albumin Immobilization INTRODUCTION macrophages both in vitro and in vivo, was reported in Inflammation is part of the complex biological unique microhabitats on surfaces and internal spaces of response of vascular tissues to harmful stimuli, such as marine invertebrates. The low amounts produced from the pathogens, damaged cells, or irritants. However, if the higher marine organisms as well as the striking structural response is exaggerated, misdirected, or long term the similarities between some pharmaceutically active agents inflammation can adversely affect health and give rise to and known microbial metabolites addressed a question many conditions such as inflammatory bowel disease, about their biosynthetic origin [3]. rheumatoid arthritis, atherosclerosis, alzheimer’s, asthma, The application of statistically based experimental psoriasis and multiple sclerosis; many of these designs is a must to deal with the effect of different inflammatory diseases are becoming common throughout culture variables in the same time. The Plackett-Burman the world [1]. designs are powerful tools for searching the most There was a remarkable increase in marine anti- significant factors for bioactive compounds production inflammatory pharmacology research nowadays. The [4]. Immobilization techniques mostly applied for the molecular mechanism of action of several marine natural production of secondary metabolites through cell products, which were shown in preclinical entrapment and cell adsorption on solid supporters. pharmacological studies to target neutrophils and The application of immobilized cells to study microbial several publications [2]. Bacteria are regularly observed in