Does propofol or caffeic acid phenethyl ester prevent lung injury after hindlimb ischaemia-reperfusion in ventilated rats? Ahmet Akyol a, * , Hu ¨lya Ulusoy a , Mustafa I ˙ mamog ˘lu b , Ali C ¸ay b , Esin Yulug ˘ c , Ahmet Alver d ,EnginErtu¨rk a , Mu¨geKos ¸ucu a , Ahmet Bes ¸ir a , Abdurrezak Akyol e ,I ˙ brahim O ¨ zen a a Department of Anesthesiology and Critical Care, Karadeniz Technical University, Faculty of Medicine, 61080 Trabzon, Turkey b Pediatric Surgery, Karadeniz Technical University, Faculty of Medicine, 61080 Trabzon, Turkey c Histology and Embriology, Karadeniz Technical University, Faculty of Medicine, 61080 Trabzon, Turkey d Biochemistry, Karadeniz Technical University, Faculty of Medicine, 61080 Trabzon, Turkey e Veterinary Surgeon, Mac ¸ka Vocational School, Trabzon, Turkey Accepted 3 January 2006 Injury, Int. J. Care Injured (2006) 37, 380—387 www.elsevier.com/locate/injury KEYWORDS Propofol; Caffeic acid phenethyl ester; Ischaemia-reperfusion; Lung injury; Rat Summary Background: To investigate the effects of propofol and caffeic acid phenethyl ester (CAPE) on prevention of lung injury as a remote organ after performing hindlimb ischaemia-reperfusion (IR) in a rat model. Methods: The animals were divided randomly into one of four groups: sham, no IR (n = 8), control, IR, (n = 8), CAPE group, IR with CAPE, (n = 8), propofol group, IR with P, (n = 8). After the rats were anaesthetised, the animals in the CAPE group received CAPE of 10 mmol, in the propofol group received propofol 50 mg/kg, in the control group received a similar volume of saline solution by means of intraperitoneal injection 1 h before reperfusion. After 4 h of ischaemia the tourniquet was removed and the animals were released for reperfusion for 4 h thereafter. At the end of the reperfusion period, a median sternotomy was performed. A blood sample was obtained for plasma malondialdehyde (MDA). The lung tissues were also removed for MDA assays, myeloperoxidase (MPO) activity, and histopathological examination. Results: Plasma and lung MDA levels, and lung MPO activity were significantly higher in the control group compared to the other groups ( p < 0.0005). In the CAPE group, * Corresponding author at: Department of Anesthesiology and Critical Care, Karadeniz Technical University, Faculty of Medicine, 61080 Trabzon, Turkey. Tel.: +90 533 633 13 69; fax: +90 462 325 22 70. E-mail address: ahmetmelike@yahoo.com (A. Akyol). 0020–1383/$ — see front matter # 2006 Elsevier Ltd. All rights reserved. doi:10.1016/j.injury.2006.01.004