Journal of Environment and Earth Science www.iiste.org ISSN 2224-3216 (Paper) ISSN 2225-0948 (Online) Vol.4, No.8, 2014 50 Adsorption of Malachite Green and Methyl Green on Cow bone Dler. M. Salh Chemistry Department, Faculty of Science and Educational Science, Sulaimaniyah University, Kurdistan Region, Iraq Corresponding Author E-mail: dlerchem@yahoo.com Abstract The animal bone meal prepared from the Cow bone has been investigated as a low cost solid adsorbent used for the removal of the hazardous Malachite Green (MG) and Methyl Green (MeG) from wastewater. It was proved that the concentration used was 200ppm as adsorption of MG and MeG dyes. The study was carried out in a batch system to optimize operation variables: working temperature, time and pH for the same adsorbent quantity of bone ash dye solution. This procedure is particularly suitable and has low cost system for purification of wastewater from MG and MeG at relatively short period of time. Keywords: wastewater, Animal (Cow) Bone, Malachite Green, Methyl Green, Spectrophotometer. 1. Introduction: The major point sources of pollution originate from the collection and discharge of domestic wastewaters, industrial wastes (Deborah Chapman 1996). The textile dyeing industry consumes large quantities of water and produces large volumes of wastewater. Wastewater from printing and dyeing units is often rich in color, containing residues of reactive dyes and chemicals ( Zongping Wang et al. 2011). Wastewater management is one of the challenging issues in the world. The traditional aim of wastewater treatment is to enable wastewater to be disposed safely. Increasingly another important aim of wastewater treatment is to recover energy, nutrients, water and other valuable resources from wastewater (Dr. Michael R. Templeton; Prof. David Butler 2011). Malachite green (MG), is the Triphenylmethane dyes it is related to some of the oldest synthetic cationic dyes, it is still used in large quantities for coloring tin-weighted silk, wool, and paper, while methyl green, (MeG), is the nitro derivative of methylene blue, is an interesting dye, also it is used chiefly for producing black shades on silk, in combination with logwood-iron mordants and also with tin phosphate.( Klaus Hunger 2003 & H. E. Fiera et al. 1949). Many materials, like fly ash and walnut shell activated carbon silicates and porous glass, etc, where used as adsorbents for adsorb wastewater contaminants (A.A. Nazari Moghaddam et al. 2010 & Y.C. Sharmai et al.2009). Literatures were also shows that MG can be eliminated using another different techniques, like; ultrasound irradiation (C. Berberidou et al.2007 & Hazrat Ali et al.2009),, Biosorption using (bacterial cellulose) ( Ali Ashjaran1 et al 2012), Enteromorpha carbon and Hydrilla verticillata biomass i ((Yijie Chen et al 2012, R.Jayaraj§ et al 2011, Rajesh Kannan, R. et al 2010, C.Parvathi et al 2011, B. Cheknane et al 2010, Hajira Tahir et al 2010, A. Bennani Karim et al 2011, Seema Singh et al 2013, P T Godbole and A D Sawant 2006, R. Rajeshkannan et al 2010, Rais Ahmad and Rajeev Kumar 2010, Mi-Hwa Baek et al 2010 & Xiangliang Pan, Daoyong Zhang 2009). Among the above, bone reflects a remarkable affinity for pollutants. These pollutants become bound up in the adsorbent through the process of adsorption (R. Slimani et al 2011 & M. El Haddad et al 2012). In the present work, a cow bone was chosen as a cheap material for the purpose of elimination of one of the two types of dyes which are Malachite Green and Methyl Green 2. Materials and Experiments: Bones ash sample was taken from Animal (Cow), was cleaned from the residual meat and muscles and finally grinded then combusted at 800 oC for 3 hours in the muffle furnace. The bone ash was cooled in a dissector and crashed with sieving; choosing the mesh size of 63µm. Purified MG and MeG (99.99%) were used as standards. Uv-vis spectra were recorded using TU-1800S UV-Vis spectrophotometer. The pH of the solutions was adjusted above 4 for MG and for MeG between 5-7. The calibration curve of MG and MeG was obtained using series standard solutions of MG and MeG, and the absorbance of both was measured at 617nm and 630nm respectively.