International Journal of ChemTech Research CODEN( USA): IJCRGG ISSN : 0974-4290 Vol.1, No.4, pp 1079-1086, Oct-Dec 2009 Development of HPTLC method for estimation of Wedelolactone, Quercetin and Jatamansone in Polyherbal Formulation R. M. Thorat*, V. M. Jadhav, V. J. Kadam, S. S. Kamble And K. P. Salaskar Department of Quality Assurance, Bharati Vidyapeeth’s College of Pharmacy, Sector 08, CBD Belapur, Navi-Mumbai – 400 614, India. *E-mail: drvmjadhav_bvcop@rediffmail.com, rupali.78@rediffmail.com ABSTRACT: In the present study an attempt has been made to develope a new, simple, sensitive, precise and robust High-performance thin layer Chromatographic [ HPTLC ] method for the quantitative estimation of Wedelolactone, Quercetin and Jatamansone in polyherbal formulation. Analysis of Wedelolactone, Quercetin and Jatamansone were performed on TLC aluminium plates pre-coated with silica gel 60F-254 as stationary phase. Linear ascending development were carried out in twin trough glass chamber saturated with mobile phase consisting of Toluene : Acetone : Formic acid [ 11: 6 : 1 v/v ] for Wedelolactone, Toluene : Ethyl Acetate : Methanol [ 4.4 : 5 : 0.6 v/v ] for Quercetin and Petroleum-ether : Acetone [ 3 : 1 v/v ] for Jatamansone at room temperature [ 25 ± 2 ° C ]. Camag TLC scanner III was used for spectrodensitometric scanning and analysis of the plate in absorbance mode at 366 nm for Wedelolactone, 254 nm for Quercetin, 285 nm for Jatamansone. The system were found to give compact spots for Wedelolactone [ Rf = 0.56], Quercetin [ Rf = 0.47 ] and Jatamansone [ Rf= 0.34 ]. The data for calibration plots showed good linear relationship with r 2 = 0.9944 for Wedelolactone, r 2 = 0.9985 for Quercetin and r 2 = 0.9975 for Jatamansone in the concentration range of 500-2500 ng/spot, 3000-8000 ng/spot and 2000-6000 ng/spot with respect to peak area. Wedelolactone, Quercetin and Jatamansone were found to be 1.62%, 0.24% and 0.11% respectively in Polyherbal Oil Formulation. The present method were validated for accuracy and recovery. The limits of detection and limits of quantification were determined. Statistical analysis of the data showed that the method is reproducible and selective for estimation of Wedelolactone, Quercetin and Jatamansone. Key-words : High-performance thin layer chromatography, Wedelolactone, Quercetin, Jatamansone. INTRODUCTION Bhavaprakash, an Ayurvedic treatise mentions the use of drug for the treatment of “ Indralupta ” i. e Drug used in the treatment of hair loss. Eclipta alba Hassk [ Family Asteraceae ], Hibiscus rosa sinensis Linn [ Family Malvaceae ], Nordostachys jatamansi DC [ Family Valerianaceae ] is such herb with traditional claims of *Corres.author: Mrs. Rupali M. Thorat. M.Pharm Student Bharati Vidyapeeth’s College of Pharmacy, Sector 8, CBD Belapur, Navi Mumbai. E-mail:drvmjadhav_bvcop@rediffmail.com , rupali.78@rediffmail.com Telephone No. : (022) 27882901, 09766533037 hair growth promotion 1 . Eclipta alba Hassk [ Family Asteraceae ], is small much- branched annual herb with white flower heads found in moist situation through-out India ascending upto 600 feet, grows just after the first showers of rainy season. It contain coumestan derivatives: Wedeloactone and demethylwedelolactone 2 . Wedelolactone is responsible for hair growth activity 3 . It is used for hair growth promoter, improving the luster of the hair, treatment of variety of human ailments, particularly liver disorders and wound healing 4 . The herb Hibiscus rosa-sinensis Linn [Family Malvaceae] is a glabrous shrub widely cultivated in the tropics as an ornamental plant and has several forms with varying colour of flowers. It contain anthocyanins and flavonoids; cyanidin-3, 5-diglucoside, cyanidin-3- sophoroside-5-glucoside,quercetin-3,7-diglucoside,