Poster Presentations S61 investigate the influence of the hepatitis B virus X protein on function of natural killer (NK) cell in NK-92 cells. Methods: The recombinant eukaryotic expression plasmid pcDNA3.1 (+)-HBX was transfected into NK-92 cells with lipofectamine. The expression of HBV X gene was detected by RT-PCR and Western blotting. Western blotting was also applied for the determination of NKG2D level in NK-92 cells. ELISA was employed to determine the IFN-g level secreted by NK-92 cells. And finally the cytotoxicities of NK cells were analyzed by MTT colorimetry, with the hepatoblastoma cell line (HepG2) as target cell. Results: RT-PCR and western blotting confirmed the expression of HBV X gene in the NK-92 cells transfected with pcDNA3.1(+)-HBX. Compare to empty vector transfected and untransfected cells, NKG2D level significantly decreased, cytotoxicity function and IFN-g secretion markedly attenuated in NK-92 cells transfected with cDNA3.1(+)- HBX. Conclusions: Transient expression of HBV X gene can decrease IFN-g secretion and cytotoxicities of NK-92 cells. The influence of the hepatitis B virus X protein on cytotoxicities of NK cell was probably associated with downregulation of expression of NKG2D. PP-119 Lamivudine in hepatitis B reactivation in patients with rheumatologic diseases on chronic immunosuppression M.E. Velasquez 1 , A. Salvana 1 , E. Tripon 1 , E.L. Ang 1 , N. Estanislao IV 1 , H. Flores 1 , V. Banez 1 , J. Ong 1 . 1 University of the Philippines Philippine General Hospital, Philippines Introduction/Objectives: Chronic immunosuppression is a mainstay of treatment for rheumatologic diseases, but has been associated with hepatitis B reactivation. This review focused on the effectiveness of lamivudine as prophylaxis or treatment of hepatitis B reactivation among patients with rheumatologic diseases on chronic immunosuppression. Methods: A search of Medline, Pubmed and Cochrane databases was performed which yielded 18 studies including 4 observational cohort studies (1 prospective, 3 retrospective). Case reports and reviews were excluded. Authors were contacted to get fulltext articles. Results: Forty-four rheumatologic patients on prednisolone alone or in combination with DMARDS or biologics, received lamivudine 100 mg/day as treatment (n = 22) for reactivation or as prophylaxis (n = 22). One study included 5 Lupus Nephritis patients while the 3 studies included patients with Rheumatoid Arthritis (n = 14), Systemic Lupus Erythematosus (n = 6), Ankylosing Spondylitis (n = 4), Polymyalgia Rheumatica (n = 5), Psoriatic Arthritis (n = 3) and 1 patient each with Systemic Sclerosis, Sjogren’s Syndrome, Dermatomyositis/Polymyositis, Takayasu Arteritis, Henoch- Schonlein Purpura and Behcet’s syndrome. Elevated levels of alanine transferase (ALT) at baseline (n = 22) normalized shortly after lamivudine therapy. HBV-DNA levels were significantly suppressed in 17 patients after treatment. Two patients developed treatment-resistant YMDD mutation of HBV and had to be shifted to adefovir. There were no major adverse events reported and lamivudine treatment appeared safe and well-tolerated. Conclusion: Lamivudine as treatment and prophylaxis for hepatitis B reactivation is a promising strategy in rheumatologic patients on chronic immunosuppression. However, the studies are limited by small sample sizes and heterogeneity, in terms of the type of rheumatologic disease, type and dosage of immunosuppressive drugs, and duration of lamivudine treatment. There is a need for further prospective, preferably RCTs including a larger set of patients. PP-120 HBV contamination of medicine instruments in surgery department M.M. Attarpour Yazdi 1 *. 1 Department of Microbiology, Faculty of Medicine, Shahed University, Tehran, Iran Objectives: Increase in occurrence of HBV (Hepatitis B Virus) among the medicine staffs and 3% prevalence of this infection in Iran and existence of the HBsAg positive individuals without any special symptom lead to general consideration about transmission of this infection by medicine instruments. Methods: The purpose of this study was evaluation of HBV contamination in surfaces (such as cabinet and door handles, telephones, water valves and electrical buttons, ... ) and equipments in the surgery department of the Cina hospital on 2009. Sampling was performed with sterile cotton swabs in transport medium (BSAS: Bovine Serum Albumin in Sodium chloride). Samples were tested by PCR technique. Results: As a result, 43.3% (13 out of 30 samples) of surfaces and 27.2% (25 out of 92 samples) of equipments were contaminated before disinfection. 16% (4 out of 25 contaminated samples) of equipments remained contaminated after disinfections. Conclusion: There is high contamination percentage in the surfaces that expresses the necessity of effective and regulatory disinfection procedures in these sites. According to the high level of infection in the surfaces and equipments in the surgery department, these approaches to disinfect equipments are not sufficient to omit HBV infection. PP-121 A case control study on the relationship between IL-6 572, RANTES genetic polymorphisms and susceptibility to the chronic hepatitis B among Han adults F.M. Feng 1 *, B.X. Gao 1 . 1 Department of Preventive Medicine; North China Coal Medical University, China Objectives: To investigate the association between the gene polymorphism of IL-6 and RANTES and the outcome of chronic hepatitis B among in Tangshan area. The research of genetic polymorphisms improved the understand of environment-individual susceptibility interaction in molecular machine and explored the risk factors of chronic hepatitis B form environmental and the genetic polymorphisms of IL-6 and RANTES. Methods: A case control study was adopted in the research, which included 118 and 61 patients with chronic and acute hepatitis B (CHB and AHB) virus infection respectively. PCR-RFLP was used to detect IL-6 and RANTES gene SNPs ( 597G, 572C of IL-6, and 403G, 28C,In1.1 T of RANTES). Information on environmental-related risk factors and pathological changes of tuberculosis was collected using a pre-tested standard questionnaire. Statistics analysis was conducted with SPSS for Windows software. Results: The sex, age, BMI had no differennce in case and control group. Drinking of man in the CHB was higher than AHB, the virus-load was also higher in the group of CHB than AHB. A special serum construction: the masculine of HBsAg, HBeAg, anti-HBcIgG and PreSAg at the same time was the hight risk for CHB. But the high level of ALT, AST, TBIL was the low risk for CHB. IL-6 572GG genotype occurred more frequently in the CHB than that in the control (c 2 = 8.627, P = 0.003), with crude OR CG = 2.024, 95% CI: 1.009 4.06; OR GG = 3.367, 95% CI: 1.169 9.709. RANTES In1.1TC genotype occurred more frequently in the CHB than that in the controls (c 2 = 6.190, P = 0.018), with crude OR TC = 2.278, 95% CI: 1.079 4.808; OR TT = 1.845, 95% CI: 0.770 4.425. There were no interaction between the gene of IL-6 572 and the index which includeALT, HBeAg and virus load.