Study on Vaccination Efficacy against Avian Influenza at Rajshahi, Bangladesh Md. Hemayatul Islam* 1 , Md. Jalal Uddin Sarder 1 , Md. Shofiqul Islam 2 , KM Mozaffor Hossain 1 and Mohammad Farhad Hossen 3 1 Department of Veterinary and Animal Science, University of Rajshahi, Bangladesh 2 Institute of Biological sciences, University of Rajshahi, Bangladesh 3 G5 Animal Science, Jessore, Bangladesh *Corresponding author’s email: hislam.islam@gmail.com Abstract Vaccines are used in integrated control strategies to guard poultry against H5N1 high-pathogenicity avian influenza. This study was to evaluate the efficacy of AI vaccine against the Avian Influenza in poultry. About 400 serum sample collected from selected 130 farms located at Rajshahi region, Bangladesh. The study was carried out from January 2013 to January, 2015. The AI vaccine titres compared with breed of layer, environmental temperature, feed and egg production. The whole experimental design was statistically tested t & F value was used for significance level. The value of titres (mean ±SD) in Hyline brown, Hyline white, Bovans white and Novogen white were 6.71±0.05, 6.67±0.06, 6.79±0.03 and 6.43±0.04. The highest (mean ±SD) serum antibody titres were 6.61±0.24 against AI antibody in >26º Environment temperature. The highest and lowest (mean ±SD) serum antibody value of AI against Feed brand (nutrient) were 7.75±0.08 & 5.58±0.22 for Aftab and Quality brand feed. 3rd WVPA Asia Meeting Prevention is better than cure 3rd WVPA Asia Meeting – Prevention is better than cure • Page 1 Copyright jointly held by the author and the World Veterinary Poultry Association 20-21st October • Manila • Philippines