Short report Anti-angiogenic effect of soybean kunitz trypsin inhibitor on human umbilical vein endothelial cells Yadollah Shakiba ⁎ , Kamran Mansouri, Ali Mostafaie Medical Biology Research Center, P.O. Box 1568, Kermanshah University of Medical Sciences, Sorkheh Ligeh, Kermanshah, I.R. Iran Received 28 September 2006; accepted 13 March 2007 Available online 25 May 2007 Abstract Soybean kunitz trypsin inhibitor (STI) was purified from aqueous extract of defatted soybean meal by affinity and ion exchange chromatography. In this study the effect of purified STI on cell migration and tubulogenesis in microcarrier-based fibrin gel was assayed. Purified STI had strong inhibitory effect on human umbilical vein endothelial cells migration and tubulogenesis in fibrin matrix, without toxic effects in the studied doses. © 2007 Elsevier B.V. All rights reserved. Keywords: Soybean trypsin inhibitor; Angiogenesis; Endothelial cell; Fibrin 1. Plant Plant material consisted of commercially available soybean seeds. Seeds were identified by one of the authors (A. Mostafaie). 2. Uses in traditional medicine Soy foods potentially have a significant health-promoting effects, including preventing and treating hypertension [1], cholesterol level reduction [2], improved vascular health [3], preserved bone mineral density [4], reduction of menopausal symptoms [5] and cancer prevention [6]. 3. Previously vhisolated classes of constituents Two protease inhibitors [7,8], lipooxigenases [9], glycinin and conglycinin [10], lectins [11], genistein and daidzein [12]. 4. Tested material Soybean kunitz trypsin inhibitor (STI) was purified from aqueous extract of defatted soybean meal by affinity column with immobilized trypsin and subsequent anion exchange chromatography on a DEAD Sepharose Fast Flow column. The Fitoterapia 78 (2007) 587 – 589 www.elsevier.com/locate/fitote ⁎ Corresponding author. Tel.: +98 831 4274617 21x208. E-mail address: shakiba@mbrc.ac.ir (Y. Shakiba). 0367-326X/$ - see front matter © 2007 Elsevier B.V. All rights reserved. doi:10.1016/j.fitote.2007.03.027