Reviews of Progress Vol -1 , ISSUE –1, May01 2013 ISSN:- 2321-3485 1 COMPARISION OF TWO DNA EXTRACTION METHODS USING THE RT-PCR FAHRI GAVAZAJ 1 , ILIA MIKEREZI 2 , BAHRIJE GAVAZAJ 2 , VALON MORINA 2 , BEKIM SAMADRAXHA 3 , FATMIR CAKAJ 3 1,3 Department of Biology, Faculty of Medical Science “REZONANCA” 2 Department of Biology, Faculty of Natural Science, University of Tirana 2 Faculty of Mathematical Natural Science, University of Pristina 2 Faculty of Mining and Metallurgy, University of Pristina 3 Faculty of Education, University of Pristina Abstract : The rapid development of molecular genetic analysis tools has made it possible to analyze most biological material even they are in a small quantity. Many different extraction protocols are being used to isolate DNA. However not all extraction methods perform equally well with heavily inhibited factors and sometimes is very important to choose an extraction procedure that could extract DNA efficiency and had the ability to minimize the amount of inhibitors co-extracted with the sample. Two different extraction methods were chosen: Chelex and Organic Extraction. To evaluate the efficiency of the procedures, quantity of DNA is compared using TaqMan Probe & Absolute Quantification method in ABI Prism RT-PCR. The samples used were blood samples. The results showed that with both methods extraction was efficiency for further analysis, but concentration of DNA was greater extracted with Chelex than it with Organic Extraction. Compare with Organic Extraction, Chelex is more efficient, more rapid, inexpensive, involves fewer steps and thus fewer opportunities for sample to sample contamination, no hazard chemicals are used. Keywords: Extraction, DNA, Chelex, Organic Extraction, RT-PCR. INTRODUCTION Biological evidence from crime scenes often provides lower quality DNA or may even require analysis from a single cell. The extraction procedure is crucial step in the process of routine forensic human identification. A variety of DNA extraction methods has been used for forensic DNA analysis procedures. Digestion of body fluid stains using SDS and proteinase K, followed by purification of DNA by extraction with phenol/chloroform and ethanol precipitation, is very successful and is routinely used for forensic samples. This method, however, was found to have limitations when applied to a polymerase chain reaction (PCR)-based DNA typing method used in forensic analysis, specifically when this method