Announcement of population data STR data for 15 loci in a population sample from the central region of Mexico S. Herna ´ndez-Gutie ´rrez a,b , P. Herna ´ndez-Franco a , S. Martı ´nez-Tripp a , M. Ramos-Kuri a , H. Rangel-Villalobos c, * a Laboratorio de Biologı ´a Molecular, Universidad Panamericana, Me ´xico DF, Mexico b Departamento de Medicina Geno ´mica y Toxicologı ´a Ambiental (IIB-UNAM) 2 , Me ´xico DF, Mexico c Laboratorio de Gene ´tica Molecular (CUCie ´nega), Universidad de Guadalajara, Departamento de Ciencias Ba ´sicas, Ocotla ´n, Jalisco, Me ´xico, Mexico Received 30 July 2004; accepted 8 September 2004 Available online 30 October 2004 Abstract A Mexican population sample was obtained from the central region of the country, including five states. Two hundred and eleven individuals were PCR-typed for 15 STR loci with the AmpFi STR Identifiler PCR amplification kit (Applied Biosystems). The following autosomal markers were analyzed: D8S1179, D21S11, D7S820, CSF1PO, D19S433, vWA, TPOX, D18S51, D3S1358, TH01, D13S317, D16S539, D2S1338, D5S818, FGA and Amelogenin. Allele frequencies for each STR were estimated and compared to previous reports. Genotype distribution by locus and by two loci combination was in agreement with Hardy–Weinberg expectations for all 15 STRs. This STR system in Mexican-mestizos presented a combined probability of exclusion (PE) and discrimination (PD) longer than 99.999%. # 2004 Elsevier Ireland Ltd. All rights reserved. Keywords: STR; Allele frequencies; Forensic; Mexico 1. Population Unrelated individuals implicated in parentage tests from the central region of Mexico (states of Mexico, Morelos, Queretaro, Puebla and Distrito Federal). 2. Extraction DNA from whole blood, body fluids and buccal cell was purificated with PUREGENE DNA purification kit from Gentra systems and phenol chloroform method. 3. PCR The AmpFiSTR 1 Identifiler TM PCR amplification kit (Applied Biosystems, Foster city, CA) was used according to the manufacturer’s instructions, using 1–2 ng of DNA tem- plate in a 25 mL PCR total volume. After a pre-incubation step at a 95 8C 11 min, amplifications were carried out in a Perkin-Elmer model 2400 thermal cycler during 28 cycles. PCR conditions were the following: 94 8C for 1 min, anneal- ing at 59 8C for 1 min, extension at 72 8C for 1 min and a final extension for 60 min at 72 8C. 4. Typing Samples were analyzed using the ABI Prism TM 310 Genetic analyzer (Applied Biosystems). Capillary electro- www.elsevier.com/locate/forsciint Forensic Science International 151 (2005) 97–100 * Corresponding author. Tel.: +392 92 50026; fax: +392 92 53099. E-mail address: hrangel@cuci.udg.mx (H. Rangel-Villalobos). 0379-0738/$ – see front matter # 2004 Elsevier Ireland Ltd. All rights reserved. doi:10.1016/j.forsciint.2004.09.080