relation between tumors with extensive pS2 staining and tumors with less intense staining in terms of lymph node metastasis (Chi-square, p = 0.041, Kendall Tau: p = 0.024, r = –0,373). In the R0 resection group, in uni- variate analysis, we found that with higher scores of HSP-70 staining, the prognosis of the patient tended to improve. (Cox regression, p = 0.013). In multivariate analysis, HSP-70 expression was found to be an inde- pendent prognostic factor. We found no relationship be- tween pS2 staining and patient survival. Key words: HSP-70 – pS2 – Pancreatic adenocarcinoma. Introduction It has generally been recognized that curative surgical resection is the only clinical parameter related to a fa- vorable prognosis in pancreatic adenocarcinoma. Prog- nostically relevant factors based on the pathological as- sessment of the resected pancreas, such as T and nodal stages, tumor grade, lymph vessel and perineural inva- sion and resection margins, were found to influence pa- tient survival in a number of studies [1, 2, 8, 15, 23, 24]. It is possible that the fate of patients with resectable car- cinomas is determined by tumor-biological factors. Sev- Summary We investigated pS2 and HSP-70 protein expression in 36 pancreatic adenocarcinomas for their effect on dis- ease extent and patient outcome. The cases were re- viewed, histologically diagnosed, typed, graded, and staged. Lymphatic vessel, blood vessel and perineural invasion as well as lymph node, resection margin and adjacent organ involvements were re-evaluated. The standard streptavidin biotin immunperoxidase method was used for immunostaining with pS2 and HSP-70 an- tibodies. Cytoplasmic staining with both antibodies was scored semiquantitatively. The scores were compared with histopathological prognostic parameters using sta- tistical methods. Standard prognostic parameters and staining scores were tested by survival analysis in terms of their effect on survival. All the tumors showed a positive cytoplasmic reac- tion with HSP-70 antibody. Seventy-seven percent of the tumors showed positive cytoplasmic staining with pS2 antibody (22.2% +, 13.9% ++ and 41.7% +++). There was a statistically significant difference between HSP-70 staining scores with N status and final stages of the tumors (Chi-square, p = 0.03 and p = 0.026, respec- tively), while neither direct nor inverse correlation was detected for both parameters. PS2 staining scores showed no statistically significant relationship with tumor grade T, M status, perineural invasion, lymph and blood vessel invasion. In tumors with extensive staining with pS2, tumor stage tended to be low (Chi square, p = 0.024, Kendall Tau-b, r: –0.336, p = 0.036). There was a statistically significant difference and inverse cor- Original Paper Immunohistochemical Detection of PS2 Protein and Heat Shock Protein-70 in Pancreatic Adenocarcinomas. Relationship with Disease Extent and Patient Survival Özgul Sagol 1 , Burçin Tuna 1 , Ahmet Çoker 2 , Sedat Karademir 2 , Funda Obuz 3 , Hüseyin Astarcıog ˇ lu 2 , Ali Küpeliog ˇ lu 1 , I · brahim Astarcıog ˇ lu 2 , Ömer Topalak 4 1 Department of Pathology, 2 Department of Surgery, 3 Department of Radiology, and 4 Department of Gastroenterology, Dokuz Eylül University Hospital, I · zmir, Turkey Pathol. Res. Pract. 198: 77–84 (2002) 0344-0338/02/198/2-77 $15.00/0 PATHOLOGY RESEARCH AND PRACTICE © Urban & Fischer Verlag http://www.urbanfischer.de/journals/prp Address for correspondence: Özgul Sagol, Dokuz Eylül Üniversitesi Tıp Fakültesi, Patoloji ABD, 35340, I · nciraltı/ I · zmir, Turkey. Tel: +90-232/277-7777-3407; Fax: +90-232/277-7274; E-mail: sagolo@deu.edu.tr