Theor Appl Genet (1991) 82:174-178 ; ~ !,~ ~I~I~NET CS 9 Springer-Verlag 1991 RFLP-based genetic map of rye (Secale cereale L.) chromosome 1R M.L. Wang, M.D. Atkinson, C.N. Chinoy, K.M. Devos, R.L. Harcourt, C.J. Liu, W.J. Rogers and M.D. Gale* Cambridge Laboratory, Colney Lane, Norwich NR4 7UJ, UK Received September 20, 1990; Accepted January 8, 1991 Communicated by J.W. Shape Summary. A map of chromosome 1R of rye was con- structed using 16 molecular and biochemical loci. From long arm to short arm, known-function loci were placed in the order: XAdh - XLee - Glu-Rl[Sec-3] XPpdk-lR - XEm-IR-1 - XEm-IR-2 - Centromere - J(Nor-R1 - Gpi-R1 - XGli-Rl[Sec-la] along with six anonymous genomic and cDNA clones from wheat. The map, which spans 106 cM with 12 loci clustered in a 15-cM region around the centromere, shows reasonably good agree- ment with previously published maps for the centromeric region, whereas the XNor-R1 - Gpi-R1 region gives a much larger distance than previously reported. Key words: Rye - Genetic mapping - RFLP - Storage protein - Isozyme et al. 1984), yellow rust, Puccinia striiformis Westend., I5-9 (Mettin et al. 1973; Zeller and Fuchs 1983) and greenbug, Schizaphis graminum Rond., Gb (Wood et al. 1974). Further manipulation of 1BL.1RS by induced homoeologous recombination (Koebner and Shepherd 1986; Koebner et al. 1986), in order to maintain the yield advantage while avoiding the quality penalty, will require well-mapped short arms of IR and lB. Our interest in the genetic map of rye is both to provide the means of manipulating the introgression of rye chromosome fragments into wheat and, since it is a simple diploid Triticeae species, as a prelude to mapping the more complex hexaploid bread wheat, Triticum aes- tivum L. (2n = 6x = 42). Introduction Rye, Secale cereale L. (2n=2x=14), is of importance both as one of the major cereal crop species, and as the donor of a component genome of the synthetic cereal, triticale. In wheat breeding, rye is also important as a donor of chromosome segments carrying useful agro- nomic genes. Probably the most significant wheat-rye introgression to date is the 1BL.1RS translocation that appears to have yield-enhancing properties (Rajaram et al. 1983), albeit with detrimental effects on bread-mak- ing quality (Zeller et al. 1982). In addition, 1RS has been shown to carry genes for resistance to powdery mildew, Erysiphe graminis DC., Pm8 (van Kints 1986), stem rust, Puccinia graminis Pers., Sr31 (Hu and Roelfs 1986), leaf rust, Puccinia recondita Rob. ex Desm., Lr26 (Bartos * To whom correspondence should be addressed Material and methods Genotypes Sixty F z plants or bulk F 3 progenies derived from individual F2s from the cross between the experimental Polish inbred lines, DS2 and RxL, supplied by Dr. P. Masoj6, Academy of Agricul- ture, Szczecin, were characterized and used for linkage analysis. RFLP markers Nine known-function and five anonymous wheat cDNAs, and four anonymous genomic wheat DNA clones were used as probes for RFLP analysis (Table 1). All these clones contain sequences which, when used as probes, cross-hybridize to rye genomic DNA. The anonymous cDNA and gDNA clones had previously been shown to be homologous to sequences on wheat group l chromosomes. The homoeologous arm locations in wheat had been ascertained by hybridization to the appropriate Chinese Spring ditelosomic lines (cDNAs: S. Chao, P. J. Sharp and M. D. Gale, unpublished results; gDNAs: R. L. Harcourt and M. D. Gale, unpublished results). The source references for information on known-function clones are given in Table 1.