Mild preeclampsia happened in 3(0.24%) patients in the NPAPP-A group and none in the LPAPP-A group. Conclusion: Treatment with LMWH and low-dose aspirin in high-risk patients with LPAPP-A improves clinical outcomes overall and in com- parison to high-risk patients with NPAPP-A. There were no differences in incidence of pPROM, fetal demise, preeclampsia, gestational age at birth, and incidence of IUGR between LPAPP-A and NPAPP-A patients. More importantly all such outcomes were significantly lower than previously reported in international literature in cohorts of low-risk patients with LPAPP-A. P1.104-N. THE EXPRESSIONS OF NUCLEAR FACTOR (ERYTHROID-DERIVED 2)-LIKE 2 (NRF2) AND PEROXIREDOXIN 6 (PRDX6) PROTEINS IN NORMAL AND PATHOLOGIC RAT AND HUMAN PLACENTAS Nuray Acar, Hakan Soylu, Imren Edizer, Ozlem Ozbey, Ismail Ustunel Akdeniz University Faculty of Medicine Department of Histology and Embryology, Antalya, Turkey Objectives: Pregnancy complications such as pre-eclampsia (PE) and in- trauterine growth restriction (IUGR) are characterized by impaired inva- sion of extravillous trophoblast and is resulted in impaired uteroplacental blood flow, contributing to placental hypoxia and oxidative stress (OS). We aimed to detect distribution pattern and expression levels of OS activated transcription factor nuclear factor erythroid 2-related factor-2 (Nrf2) and a unique antioxidant enzyme, Peroxiredoxin 6 (Prdx6) in rat control and dexamethasone induced intra-uterine growh retarded (IUGR) placentas; human control, IUGR and PE placentas. Methods: Term placentas from healthy women (control) (n¼6), women with PE (n¼6) and IUGR (n¼6) were obtained after caesarean section. Female Rattus norvegicus rats were subcutaneously injected with a dose of 100 mg dexamethasone in 0.1 ml 10% ethanol on day 13 of pregnancy. The animals subsequently received daily injections of 200 mg/kg dexametha- sone on days 14-19 of pregnancy. Control animals were injected with 0.9% saline solution on corresponding days of pregnancy. Eight control and eight IUGR group rats were sacrified on day 20 of pregnancy. Placentas were processed for immunohistochemistry and Western blotting. Results: Nrf2 and Prdx6 immunoreactivities were weaker in rat and hu- man IUGR placentas compared to rat and human control placentas, respectively. Western blotting confirmed decrease of Nrf2 and Prdx6 in rat and human IUGR placentas compared to respective control placentas with statistically significant difference. Expression of Nrf2 and Prdx6 were higher in PE placentas compared to control placentas but difference wasn’t statistically significant. Conclusions: According to our results Nrf2 was unable to rise sufficiently hence Prdx6 levels were lower in rat and human IUGR placentas. Since OS is an imbalance between reactive oxygen species (ROS) and antioxidant defenses to scavenge those species, inadequate Prdx6 production might have a role in generation of OS and pathogenesis of IUGR but not in the pathogenesis of PE. P1.105. IGF-II ANALOGUE EFFECTS ON PLACENTAL EFFICIENCY AND FETAL GROWTH IN NORMAL AND FGR MICE Jayne Charnock, Mark Dilworth, John Aplin, Melissa Westwood, Colin Sibley, Ian Crocker The University of Manchester, Manchester, UK Objectives: Enhancing placental IGF availability appears an attractive strategy for improving outcomes in fetal growth restriction (FGR). We hypothesised that one approach may be to use Leu 27 IGF-II, an IGF-II analogue that binds the IGF-II clearance receptor, IGF2R, and thereby blocks endogenous IGF-II removal, resulting in enhanced bioavailability. We investigated the impact of Leu 27 IGF-II infusion into C57BL/6 (wild- type, WT) and endothelial nitric oxide synthase knockout (eNOS -/- , FGR) mice from mid-late gestation. Methods: 1mg/kg/day Leu 27 IGFII was delivered via subcutaneous mini- osmotic pump from E12.5-E18.5 (C57 n¼23 pregnancies, 178 fetuses; eNOS n¼11 pregnancies, 74 fetuses) or vehicle (C57 n¼22, 166 fetuses; eNOS -/- n¼11, 86 fetuses). Fetal and placental weights recorded at E18.5 were used to generate frequency distribution curves; fetuses below the 5 th centile were deemed small for gestational age (SGA). Results: In WT pregnancies, Leu 27 IGFII treatment halved the number of SGA fetuses (10 vs. 5), and increased the number around the mean weight (131 v 112 pups within 1 S.D, see figure). Mixed-models analysis confirmed that Leu 27 IGFII preferentially improved fetal weight in the smallest fetuses. Placental weights were decreased in the Leu 27 IGFII treated group (81.3 ±1.3mg treated v 85.6 ±1.9mg vehicle; p<0.05), resulting in a greater fetal:placental weight ratio (p<0.05), indicative of enhanced placental ef- ficiency. Unidirectional 14C MeAIB transfer per g placenta (measure of System A amino acid transporter activity) was inversely correlated with fetal weight in Leu 27 IGFII treated animals (p<0.01). In eNOS -/- mice, Leu 27 IGFII also reduced the number of SGA fetuses (5 vs. 1). However, placental weights remained unchanged. Conclusion: Although smaller, the placentas of Leu 27 IGFII-treated C57 mice have greater capacity for supporting optimal fetal growth. Reduction in SGA pups in C57 and eNOS -/- litters supports the use of this analogue as a means of rescuing fetal growth in the smallest animals. Mechanisms un- derlying its differential effect on placental weight are under investigation. P1.106-N. FOETAL AND UMBILICAL VASCULAR REACTIVITY IN A MODEL OF IUGR THROUGH GRADUAL UTERINE ARTERY OCCLUSION IN GUINEA PIGS Daniela Scheneider a , Ren e Alegría a , Emilio Herrera c , Marcelo Farías a , Paola Casanello a, b , Bernardo Krause a a Division of Obstetrics and Gynecology, School of Medicine, Pontificia Universidad Cat olica de Chile, Santiago, Chile; b Division of Pediatrics, School of Medicine, Pontificia Universidad Cat olica de Chile, Santiago, Chile; c Laboratorio de Funci on y Reactividad Vascular, ICBM, Universidad de Chile, Santiago, Chile IUGR relates with altered placental vascular reactivity and increased car- diovascular risk in the neonate, however whether vascular changes observed in umbilical arteries reflects the alterations present in systemic arteries in the IUGR foetus in unknown. Objectives: In pregnant guinea pigs we aimed to determine the effect of gradual occlusion of uterine artery on foetal growth, and the NO-depen- dent relaxation in umbilical and systemic (aorta) arteries in IUGR foetuses. Methods: IUGR was induced by implanting ameroid constrictors in uterine arteries at 40 days of gestation. At day 63 foetuses were extracted, weighted and dissected. NO-dependent vasoactive responses to SNP (NO donor), and insulin or acetylcholine in presence or absence of L-NAME (NOS inhibitor) and BEC (arginase inhibitor) were studied by wire Ă Abstracts / Placenta 35 (2014) A1eA112 A43