Research Article
Increased Serum Interleukin-9 Levels in Rheumatoid
Arthritis and Systemic Lupus Erythematosus: Pathogenic Role or
Just an Epiphenomenon?
Andréa Tavares Dantas,
1,2
Claudia Diniz Lopes Marques,
2
Laurindo Ferreira da Rocha Junior,
1,2
Mariana Brayner Cavalcanti,
1
Sayonara Maria Calado Gonçalves,
1
Pablo Ramon Gualberto Cardoso,
1
Henrique de Ataide Mariz,
1,2
Moacyr Jesus Barreto de Melo Rego,
1
Angela Luzia Branco Pinto Duarte,
2
Ivan da Rocha Pitta,
1
and Maira Galdino da Rocha Pitta
1
1
Laborat´ orio de Imunomodulac ¸˜ ao e Novas Abordagens Terapˆ euticas (LINAT), N´ ucleo de Pesquisas em Inovac ¸˜ ao Terapˆ eutica Suely
Galdino (Nupit SG), Universidade Federal de Pernambuco (UFPE), Avenida Professor Moraes Rego, s/n, 50670-901 Recife, PE, Brazil
2
Servic ¸o de Reumatologia, Hospital das Cl´ ınicas, UFPE, Avenida Professor Moraes Rego, s/n, 50670-901 Recife, PE, Brazil
Correspondence should be addressed to Maira Galdino da Rocha Pitta; mgrpitta@gmail.com
Received 20 February 2015; Revised 4 May 2015; Accepted 5 May 2015
Academic Editor: George Perry
Copyright © 2015 Andr´ ea Tavares Dantas et al. his is an open access article distributed under the Creative Commons Attribution
License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly
cited.
he purpose of this paper was to evaluate the levels of IL-9 in patients with SLE and RA compared with controls and the association
of IL-9 levels with clinical and laboratory parameters. IL-9 levels were assessed in 117 SLE patients, 67 RA patients, and 24 healthy
controls by ELISA. Clinical and laboratory parameters were recorded. he IL-9 serum levels were signiicantly higher in RA patients
(4,77 ± 3,618 pg/mL) and in SLE patients (12,26 ± 25,235 pg/mL) than in healthy individuals (1,22 ± 0,706 pg/mL) ( < 0, 001). In
SLE patients, there were no statistically signiicant associations or correlations between the levels of IL-9 and SLEDAI or other
clinical and laboratorial parameters, with the exception of disease time, which showed a statistically signiicant negative correlation
with IL-9 levels ( = −0, 1948; = 0, 0378). In RA patients, no association or statistically signiicant correlation was observed with
disease duration, DAS28, HAQ, rheumatoid factor positivity, or erosions on radiography. hese data demonstrated increased serum
levels of IL-9 in SLE and RA patients, but further studies are needed to clarify the precise role of this cytokine and its potential use
as therapeutic target.
1. Introduction
he imbalance between efector and regulatory cell popula-
tions (Treg) is of critical importance in the pathogenesis of
various autoimmune disorders. According to the current
paradigm, the proinlammatory axis of h1 and h17 cells is
counter balanced by the cell populations h2 cells and Treg
[1].
Interleukin-9 (IL-9) is a member of the gamma-chain
family of cytokines, irst described as a member of a growing
number of cytokines that have crucial roles in the develop-
ment, proliferation, survival, and diferentiation of multiple
cell lineages of both the innate and adaptive immune systems
[2].
IL-9 production was irst associated with the h2 phe-
notype, and many of the preliminary functions of IL-9 were
tested in models of h2-associated immunity. However, it
is now known that, under speciic conditions, regulatory
(Tregs), h1, and h17 subset of T cells also express IL-9.
Recently, it has been shown that IL-9-producing CD4+ T
cells may represent the T helper subset h9 cells. In vivo
T cells produce IL-9 in both proinlammatory and anti-
inlammatory environments [3].
Hindawi Publishing Corporation
Disease Markers
Volume 2015, Article ID 519638, 6 pages
http://dx.doi.org/10.1155/2015/519638