Pareek and Arora, IJARPB, 2012; Vol.2 (3):326-337 ISSN 2277 – 6222 (Research Article) Available online on www.ijarpb.com Received on 17/05/2012; Revised on 22/05/2012; Accepted on 09/06/2012 Casein Protein: Phylogeny study of important domestic animals using computational method Pareek; K* 1 , Shah; S 1 and Asha Arora **2 1 School of life Science, Pacific University, Udaipur , 2 Head, Deptt. of Botany and Biotechnology, B.N.P.G.Girls College,Udaipur Corresponding Author: Kamlesh Pareek; School of life science, Pacific University, Udaipur kbiopareek@gmail.com ABSTRACT Casein is mammalian milk protein belong to phosphoproteins family and involved in a number of important physiological processes. We are analyzed four Subgroups of casein protein (Alpha S-1, Alpha S-2, Beta and Kappa) in four species (Cow, Camel, Domestic Sheep and Goat) reported in that family. In this study, a full length Amino acid Sequence of above four species in casein subgroup are find from public database. The candidate sequences are further analyzed by multiple sequence alignment, for phylogeny study using various tools such as Clustal X, NJ-Plot. The trees were calculated using the rooted Neighbor-Joining (NJ) method on distance matrix employing NEIGHBOR. The Default p-distance method was used for distance analysis. The results revealed that very similar relationship between AA sequences responsible for their common ancestor. Find large conserved region which is play an important role in phylogeny relationship. KEYWORD: Casein protein, multiple sequence alignment, Neighbor Joining method, conserved region, phylogeny relationship