Notch Suppresses FGF-Dependent Transformation 1 To: J. Bio. Chem., 2003 Notch Activation Suppresses FGF-Dependent Cellular Transformation Deena Small¶*, Dmitry Kovalenko¶, Raffaella Soldi, Anna Mandinova, Vihren Kolev, Radiana Trifonova, Cinzia Bagala, Doreen Kacer, Chiara Battelli, Lucy Liaw, Igor Prudovsky and Thomas Maciag† Center for Molecular Medicine Maine Medical Center Research Institute Scarborough, ME 04074 *The authors thank Drs. T. Kadesch for the Notch1 and CSL-reporter constructs, S. Artavanis- Tsakonas for the Notch2 constructs, R. Friesel for the dnFGFR1 construct, R.L. Panek for the FGFR1 inhibitor PD166866 and Norma Albrecht and Gloria Ledoux for expert administrative assistance. This work was supported in part by NIH grants CA92255 to DS, HL HL32348 and HL35627 to TM, RR15555 to TM and LL, and ACS Grant RPG97-093-03 to LL. ¶ DK and DS contributed equally to the data presented in this manuscript. * Current address: Department of Animal Nutritional and Medical Laboratory Sciences, College of Life Sciences and Agriculture, University of New Hampshire, Durham, NH † To Whom Correspondence Should Be Addressed: Center for Mol. Med., Maine Med. Ctr. Res. Inst., 81 Research Drive, Scarborough, ME 04074.Telephone: 207-885-8200; Fax: 207-885- 8179; E-mail: maciat@mmc.org Abbreviations: BCS, bovine calf serum; caN, constitutively active Notch; CSL, for CBF-1/RBP- Jk/KBF2 in mammals; Su(H) in Drosophila and Xenopus; and L ag-2 in C. elegans; dnN, dominant- negative Notch; dnFGFR1, dominant negative fibroblast growth factor receptor 1; FGF, fibroblast growth factor; FGFR, fibroblast growth factor receptor; J1, Jagged1; PBS, phosphate-buffered saline; sJ1, soluble Jagged1 Running Title: Notch Suppresses FGF-Dependent Transformation Copyright 2003 by The American Society for Biochemistry and Molecular Biology, Inc. JBC Papers in Press. Published on February 21, 2003 as Manuscript M300464200 by guest on December 2, 2015 http://www.jbc.org/ Downloaded from