*Corresponding Author Address: Dr. Asha K.R.T, Department of Biochemistry, Government Arts College, Paramakudi, Ramanathapuram District, Tamil Nadu – 623 707; E-mail: drkrtasha@gmail.com World Journal of Pharmaceutical Sciences ISSN (Print): 2321-3310; ISSN (Online): 2321-3086 Published by Atom and Cell Publishers © All Rights Reserved Available online at: http://www.wjpsonline.org/ Original Article In-vitro pharmacological applications of pigment producing halophilic microorganism from the marine region of India Asha K.R.T 1,* , Joseph Selvin 2 1 Department of Biochemistry, Government Arts College, Paramakudi, Ramanathapuram District, Tamil Nadu – 623 707 2 Department of Microbiology, Pondicherry University, Kalapet, India Received: 29-05-2016 / Revised: 06-10-2016 / Accepted: 01-11-2016 / Published: 26-11-2016 ABSTRACT In the present study, two pigment producing promising strains were identified and characterized based on the biochemical properties from the halophilic environment. Based on the biochemical and physiological characteristics the strain BQ31 and BV32 were selected and both the strains exhibited showed high similarity towards the Haloarcula species. It is identified that the strains showed wide range of carbon utilization, able to tolerate different salts with multiple antimicrobial susceptibility pattern. The extracellular pigment was identified by various chromatographic techniques and the molecular weight of the pigment was characterized by MASS spectrum. Interestingly, the pigment revealed significant liver lipid peroxidase activity and haematological parameters under different body weight of albino rats. The biochemical parameter of the pigment under different body weight of Albino rats is an additional importance. Thus the pigment could be used for the various pharmaceutical applications. Keywords: Halophilic microbes, Pigment; MS spectrum, In-vitro biological applications INTRODUCTION The microorganisms exist in extreme environments such as high salt, high temperature and high atmospheric pressures are defined as the extremophiles. These extremophilic microbes possess unique properties of biotechnological and commercial significance. They also possess special adaptation strategies that make them interesting not only for the fundamental research but also for the industrial applications [1]. Among the extrmophiles, the studies on ecology, physiology and taxonomy of halophiles have revealed an impressive diversity in hyper saline and alkaline lakes [2,3]. Halophiles are belonged to the order Halobacteriales and the family Halobacteriaceae [4]. Halophilic bacteria also categorized as psychrophilic, thermophilic, alkaliphilic, mesophilic, aerobic and anaerobic halophilic based on the environments [5]. They are mainly aerobic and exist in the hypersaline regions such as salterns, salt lakes, sub surface salt formation, and solar salts. Halophiles are mainly involved in the biogeochemistry of phosphorus, carbon and other elements in hypersaline environments [6]. Few reports claimed that halophiles also play in the degradation of organic pollutants as hydrocarbons, pesticides and crude oil [7]. In addition to degradation of organic pollutants, halophiles also used as a biocontrol agents against certain pathogenic fungi [8,9]. Traditionally, halophiles have been used in the food and nutraceutical industries as the fermentation of soy and fish sauces and β-carotene production, also, recently looked into many novel and unique molecules such as the compatible solutes, biopolymers or carotenoids, novel extracellular polysaccharides; exoenzymes such as cellulase, amylase, lipase, proteases and xylanase, and biodegradable plastics, bio-surfactants, bioemulsifiers and bacteriorhodopsins for molecular biotechnological applications [10,11]. Novel protein isolated from from Halobacterium Salinarum used in the treatment of cancer. Hypersaline environments are commonly present in the southern parts of the India. Until now, no reports on the characterization of halophilic archaeal communities from Kanyakumari district, India. The present study aimed in the isolation and identification of the halophilc bacteria from the southeast coastal region