JOBNAME: No Job Name PAGE: 1 SESS: 7 OUTPUT: Tue Mar 9 18:17:28 2010 SUM: 74B77945 /v2501/blackwell/journals/JFBC_v0_i0/jfbc_417 ANTIOXIDANT ACTIVITIES OF HIBISCUS ROSA-SINENSIS LINN. DURING 10% D-GLUCOSE FEEDING IN RAT HEART TISSUES SHANMUGHAM BHUVANA 1 *, RAMALINGAM MAHESH † and VAVA MOHAIDEEN HAZEENA BEGUM Department of Siddha Medicine Faculty of Science Tamil University Vakaiyur, Thanjavur 613 010, Tamilnadu, India Accepted for Publication July 28, 2009 ABSTRACT In the present study, the antioxidant activities of Hibiscus rosa-sinensis Linn. flower petals treatment for 4 weeks were screened in 10% D-glucose feeding in rats. Experimental rats were treated with 10% D-glucose solution to drink orally. H. rosa-sinensis flower petals (1,000 mg/kg of body weight) were mixed with normal chow diet and administrated for 4 weeks as preventive and curative effects. An appreciable decrease in peroxidation product viz. malon- dialdehyde is observed in heart tissues of H. rosa-sinensis treated in 10% D-glucose-fed rats. The decreased activities of key antioxidant enzymes such as superoxide dismutase, catalase, glutathione peroxidase, and nonenzymatic antioxidants such as glutathione, vitamin E and vitamin C in 10% D-glucose- fed rats were brought back to near-normal range upon H. rosa-sinensis flower petals treatment. In conclusion, H. rosa-sinensis exhibited preventive and curative effects in 10% D-glucose-induced oxidative stress in rat heart tissues. PRACTICAL APPLICATIONS Acute oral glucose feeding causes oxidative stress and functional decline in heart of healthy subjects. Hibiscus rosa-sinensis is one of the medicinal plants that have attracted particular attention in folk medicine because of its widespread health use around the world. This study aims to enlighten the beneficial effects of H. rosa-sinensis in 10% glucose feeding-induced heart * Present Address: Paddy Division, Indian Institute of Crop Processing Technology, Thanjavur 613 005, Tamilnadu, India. 1 Corresponding author. TEL: ••; FAX: ••; EMAIL: bhuvana.shanmugham@gmail.com † Present Address: Oriental Medicine Research Institute, College of Oriental Medicine, Dongguk University, Gyeongju 780-714, Korea. 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 DOI: 10.1111/j.1745-4514.2010.00417.x Journal of Food Biochemistry •• (2010) ••–••. All Rights Reserved. © 2010 Wiley Periodicals, Inc. 1 3 3 2