www.scholarsresearchlibrary.com t Available online a Scholars Research Library Der Pharmacia Lettre, 2016, 8 (19):57-69 (http://scholarsresearchlibrary.com/archive.html) ISSN 0975-5071 USA CODEN: DPLEB4 57 Scholar Research Library Isolation of fungal pathogen Fusarium sp. from maize kernel and its inhibition by Pseudomonas containing liquid Bioformulation Malvika Rajnandini* 1 , Rajneesh Kumar Gautam 2 , Islamuddin 3 , Nandkishor More 4 and Aakrati Sharma 5 1 Department of Environmental Microbiology, Babasaheb Bhimrao Ambedkar Central University, Lucknow, - 226025, India 2,3 Department of Civil Engeering, Babasaheb Bhimrao Ambedkar Central University, Lucknow, -226025, India 4 Department of Environmental Sciences, Babasaheb Bhimrao Ambedkar Central University, Lucknow, -226025, India 5 Banasthali University, Banasthali -304022, Rajasthan, India _____________________________________________________________________________________________ ABSTRACT Maize (Zea mays L.) is one of the most versatile emerging crops having wider adaptability under varied agro - climatic conditions. Maize has the highest genetic yield potential among the cereals. In India, about 28% of maize produced is used for food purpose , about 11% as livestock feed , 48% as poultry feed , 12% in wet milling industry (for example starch and oil production ) and 1% as seed (AICRP , 2007). Pseudomonas fluorescence, a major constituent of rhizospheric bacteria, not only encourages the plant growth through their diverse mechanisms and but also show a wider potential for biological control of different phytopathogens. This paper presents the study on the isolation and identification of fluorescent Pseudomonas and to check their bio-control potential against phytopathogen F. moniliforme and on the basis of various plant growth promoting and biocontrol attribute select the elite strain in designing of liquid based bio-formulation. Apart from this application of this Bio-formulation for its efficacy in maize growth and disease suppression is the main objective of the study. Key words: Maize, Genetic yield potential, Pseudomonas, Bio-formulation, F.moniliforme _____________________________________________________________________________________________ INTRODUCTION In developed countries, maize is consumed mainly as second – cycle produce, in the form of meat, eggs and dairy products. In developing countries, maize is consumed directly and serves as staple diet. In India, maize is the third most important food crops after rice and wheat (http://faostat3.fao.org). Global maize production has grown at a calculated annual growth rate (CAGR) of 3.4 per cent over the last ten years from 716 million metric tons (MnMT) / year in 2004-05 to 967 million metric tons / year in 2013-14. USA is the largest producer of maize in the world, followed by China and Brazil (Ranum , 2014 ). USA is also the largest exporter of maize. Production of maize in India has increased at a CAGR of 5.5 per cent from 14 MnMT in 2004-05 to 23 MnMT in 2013-14 and the areas where it is more often cultivated are Andhra Pradesh and Karnataka which contributes to ~38 per cent of the total production. India’s yield at 2.5 MT/hectare is less than half the global average of 5.5 MT/hectare. Maize consumption has increased at a CAGR of 3.6 per cent over the last five years; poultry feed accounts for ~50 per cent of maize consumption. (IMS, 2014). In India, maize is grown in a wide range of environments, extending from extreme semi-arid to sub-humid and humid regions. The crop is also very popular in the low- and mid -hill areas of