The Effect of Seed Bio-invigoration Using Indigenous Rhizobacteria to Improve Viability and Vigor of Upland Rice (Oryza sativa L.) Seeds Gusti Ayu K. Sutariati 1 *, A. Khaeruni 1 , Y.B. Pasolon 1 , Muhidin 1 , and La Mudi 1 Department of Agrotechnology, Faculty of Agriculture, Universitas Halu Oleo Kendari 93232 Southeast Sulawesi Indonesia Abstract : Seed vigor and germination ability directly affect seedling emergence and yield. Seed bio-invigoration using indigenous rhizobacteria was studied to improve viability and vigor of upland rice seeds. The research design using completely randomize design (CRD) with eighteen treatment ie. Control, Dithane, Hydration, KNO 3 + Bacillus sp. CKD061, KNO 3 + P. fluorescens TBT214, KNO 3 + Serratia sp. CMN175, NaCl + Bacillus sp. CKD061, NaCl + P. fluorescens TBT214, NaCl + Serratia sp. CMN175, matriconditioning using ground burned-rice husk + Bacillus sp. CKD061, matriconditioning using ground burned-rice husk + P. fluorescens TBT214, matriconditioning using ground burned-rice husk + Serratia sp. CMN175, matriconditioning using ground brick + Bacillus sp. CKD061, matriconditioning using ground brick + P. fluorescens TBT214, matriconditioning using ground brick + Serratia sp. CMN175, Bacillus sp. CKD061 + P. fluorescens TBT214, Bacillus sp. CKD061 + Serratia sp. CMN175, P. fluorescens TBT214 + Serratia sp. CMN175, with three replication. Research showed that seed bio-invigoration with Bacillus spp. CKD061 integrated with ground burned-rice husk or ground brick give the highest maximum growth rate, germination rate, relative growth, vigor index, and T 50 . Seed treatment with Bacillus spp. CKD061 integrated with ground burned rice husk increased vigor index by 63% when compared to control. Keywords : Bio-invigoration, Indigenous rhizobacteria, Upland Rice, Seed Viability and Vigor. Introduction Rice (Oryza sativa L.) in Indonesia is very important crop because as main staple food. Indonesia actually is major rice producing countries together with China, India, Bangladesh and Vietnam 1 , but at the same time, Indonesia also the main consumer of rice 2 . The demanding rice is expected to increase in the future, at least in line with the population growth. Although Indonesia is the third-largest country regarding global rice production, it is still a rice importer sometimes. Indonesia has the largest rice consumption per capita that reached 140 kilograms and population reached 250 million people. Considering that Indonesia has a population that consumes large quantities of rice, and facing the risks involved being a rice importer when food prices rise. International Journal of PharmTech Research CODEN (USA): IJPRIF, ISSN: 0974-4304, ISSN(Online): 2455-9563 Vol.9, No.12, pp 565-573, 2016