Effect of Biherbal Extracts of Bryophyllum calcynium and Tribulus terrestis on Biochemical Serum Changes on Ethylene Glycol Induced Urolithiasis in Female Wistar Rats P. H. MASHIYAVA 1 , S.K. RAVAL 1 , A. A. VASAVA* 2 1 Department of Veterinary Medicine, College of Veterinary Science & Animal Husbandry, Anand Agricultural University, Anand, Gujarat 2 Department of Veterinary Physiology & Biochemistry, College of Veterinary Science & Animal Husbandry, Anand Agricultural University, Anand, Gujarat *email : amritavasava@yahoo.in Advances in Life Sciences 5(20), Print : ISSN 2278-3849, 9517-9520, 2016 ABSTRACT The present research work was undertaken to study the therapeutic efficacy of biherbal extracts of Bryophyllum calcynium and Tribulus terrestis on ethylene glycol induced urolithiasis in female Wistar rats. Rats were equally distributed in five different groups. Induction of urolithiasis was done by administration of ethylene glycol at the rate of 0.75% v/v in drinking water for 21 days. Confirmation of urolithiaoisis was done by evaluating serum biochemical parameters. After confirmation of urolithiasis, rats of group IV and V were administered alcoholic biherbal extracts (1:1) at 200 mg/kg and 400 mg/kg respectively for 28 days. The blood samples from 90 Wistar rats were screened for detection of therapeutic efficacy of biherbal extracts. After 21 days of induction rats of all groups, except control group, showed significant variation in serum biochemical values.After 28 days of biherbal drug administration, rats from lithiatic control group (group II) showed significant increase in serum creatinine, uric acid, BUN and magnesium level while serum calcium level was significantly decrease as compare to normal control group. After biherbal treatment for 28 days serum profile reveals significant decrease in serum creatinine, uric acid, BUN and magnesium level while serum calcium level was significantly increase as compare to rats of lithiatic control group. Results of serum profile reveals alcoholic extract of biherbal combination of Bryophyllum calycinum and Tribulus terrestis possess good therapeutic efficacy against urolithiasis. Key words Bryophyllum calcynium, Tribulus terrestis, Ethylene glycol, Urolithiasis, Wistar rats Urolithiais or renal stone is the world’s most common ailment with maximum chance of relapse. Stones are often more commonly found in males than females due to a longer urethra (Manjula, et al., 2012). It leads to decreased productivity and higher cost of maintenance.Stones that cause obstruction to the flow of urine, set up an environment of urine stasis and bacterial growth which leads to alteration in kidney fuction tests and leads to increase Non Protinous Nitrogenous (NPN) substances (Soundararajan, et al., 2006). The treatment modalities like surgery and drug therapy are practiced in the management of kidney stones but have some limitations. Indigenous plants have been used as a potential source of medicine since ancient times. Although many plants have been evaluated for anti- urolithiatic effect, but search for medical treatment of renal calculi is still going on (Rad, et al., 2011). Monotherapy sometimes leads to failure in curing diseases having multiple aetiology and one of these problems exist in treatment of Urolithiais. Traditional medical practitioners prescribe a combination of herbal products with synergistic action. There is no single satisfactory method available for complete cure of urolithiasis. For this reason biherbal drugs are gaining popularity among practitioners for treating urolithiasis.The fruits of Tribulus terrestis are recommended for the treatment of urinary disorders and erectile dysfunction (Shirfule, et al., 2012) while anti-oxidant and nephroprotective effect of B. calcyniumis documented in various studies (Harlalka, et al., 2007). So, taking into view these points the present research work was carried out to evaluate the therapeutic efficacy of combination of biherbal alcoholic extract of Tribulus terrestis and B. calcynium at Department of Veterinary Medicine, College of Veterinary Science & A. H., AAU, Anand. MATERIALS AND METHOD The study was carried out on female Wistar rats procured from Zydus Research Center, Ahemedabad, Gujarat. Rats were adult in age having 250-300 g. weight. The experimental designing was done as described in Table No.: 1. Freshfruits of Tribulus terrestis and leaves of Bryophyllum calcynium were procured from Medicinal & Aromatic Plant Unit, Anand Agricultural University, Anand. The plant was washed thoroughly with clean water and dried in shade. The dried plant materials were powdered in electric grinder and subjected to extraction in Microwave extraction unit using alcohol following the method as described by Mandal et al., (2010). Induction of urolithiasis was done by administration of ethylene glycol at the rate of 0.75% v/v in drinking water for 21 days. Confirmation of urolithiaoisis was done by evaluating serum biochemical parameters. Administration of alcoholic extract of Bryophyllum calcynium and Tribulus terrestis was mixed in 1:1 ratio and administered by oral route using sterile 1ml syringe with oral rat gavage needle. Dose was calculated according to body weight of animal and administrated as per concentration strength of formulation. Urolithiasis induced Wistar rats were given biherbal treatment; in group IV and V were at the dose rate of 200 mg/kg and 400 mg/kg respectively, for 28 days.Blood