21 PRINT ISSN: 2394-6679 | E-ISSN: 2347-7881 Vol. 3, Issue 4 | magazine.pharmatutor.org Article Development and Validation of UV Spectrophotometric Estimation of Diclofenac Sodium Bulk and Tablet Dosage form using Area under Curve Method Mali Audumbar Digambar*, Jadhav Santosh, Mane Pandurang, Tamboli Ashpak Department of Pharmaceutics, Sahyadri College of Pharmacy, Methwade, Sangola- 413307, Solapur, Maharashtra, India *maliaudu442@gmail.com ABSTRACT A simple, precise, accurate and economical UV visible spectrophotometric method has been developed for estimation of Diclofenac sodium drug by AUC method. The standard and sample solutions were prepared by using double distilled water as a solvent. Quantitative determination of the drug was performed at wavelength range 270-282 nm. The linearity was established over the concentration range of 05, 10, 15, 20 & 25 µg/ml for Diclofenac sodium with correlation coefficient value of 0.9981. Precision studies showed that % relative standard deviation was within range of acceptable limits. The mean percentage recovery was found to be 99.38%.The proposed method has been validated as per ICH guidelines. Keywords: Diclofenac sodium, UV visible spectrophotometry, AUC, Method Validation INTRODUCTION Diclofenac Sodium (DS) is chemically Sodium salt of 2-[{2,6-dichlorophenyl}amino]benzene acetic acid. It is having anti-inflammatory and analgesic properties [1] .Literature survey revealed several analytical methods UV spectrophotometry [2] and HPLC [3] have been reported in bulk, pharmaceutical dosage form for determination of Diclofenac sodium. To our notice so far no UV- spectrophotometric method using Area Under Curve (AUC) has been reported for the determination of Diclofenac sodium in bulk and tablets. Hence an attempt has been made to develop new UV-spectrophotometry(AUC) method for estimation of Diclofenac sodium in bulk and pharmaceutical formulations with good accuracy simplicity, precision and economy. Molecular Formula: C 14 H 10 Cl 2 NNAO 2 . Molecular weight: 318.130g/mole Fig. 1:Chemical Structure of Diclofenac sodium. MATERIALS AND METHODS Apparatus and instrumentation: A shimadzu1800 UV/VIS double beam spectrophotometer with 1cm matched quartz cells was used for all spectral measurements. Single Pan Electronic balance (CONTECH, CA 223, India) was used for weighing purpose. Sonication of the solutions was carried out using an Ultrasonic Cleaning Bath (Spectra lab UCB 40,India).Calibrated volumetric glassware (Borosil®) was used for the validation study. How to cite this article: A Mali, S Jadhav, P Mane, A Tamboli; Development and Validation of UV Spectrophotometric Estimation of Diclofenac Sodium Bulk and Tablet Dosage form using Area under Curve Method; PharmaTutor; 2015; 3(4); 21-25