*
Correspondance to: Mohammad Amin Edalatmanesh ; Department of Physiology,
Islamic Azad University of Shiraz, Shiraz, Iran TEL: +98 7136191232,
Email:salehzar @gmail.com
Copyright© Asian Exercise & Sport Science Association (AESA). www.aesasport.com
International Journal of Applied Exercise Physiology
2322-3537 www.ijaep.com
Vol.5 No.4
Received: July, 2016 Accepted: November, 2016 Available online: December, 2016
The Effects of Endurance Training and Gallic Acid on BDNF and TNF-a in Male
Rats with Alzheimer
Yoones Baziyar
1
, Mohammad Amin Edalatmanesh
2*1
, Seyed Ali Hosseini
1
, Abdossaleh Zar
3
1
Department of Sport Physiology, Marvdasht Branch, Islamic Azad University, Marvedasht, Iran
2.
Department of Physiology, College of Sciences, Shiraz Branch, Islamic Azad University, Shiraz, Iran
3.
Department of Physical Education and Sport Science, Jahrom University, P.O. Box 74135-111, Jahrom, Iran
ABSTRACT:
Background: In the present study, the effects of endurance training and chronic administration
of Gallic acid (GA) on hippocampal level of brain-derived neurotrophic factor (BDNF) and
tumor necrosis factor alpha (TNF- ) in trimethyltin (TMT)-induced hippocampal degeneration
as a model of Alzheimer’s disease were investigated.
Methods: 70 male Sprague-Dawley rats were selected and randomly assigned to 7 groups:
1.control, 2.TMT, 3.TMT+GA50, 4.TMT+GA100, 5.TMT+Ex, 6.TMT+Ex+GA50,
7.TMT+Ex+GA100. For induction of Alzheimer's disease, the rats in groups 2-7 were injected
with single dose of TMT (8 mg/kg), intra-peritonealy (i.p.). Rats in the groups 5, 6 and 7 carried
out an 8-week exercise program on a motorized treadmill (15-20 m/min, 0% inclination for 15-
30 min/day, 5 days/week). In addition, the groups 3 and 6 were treated by 50 mg /kg and groups
4 and 7 are administered 100 mg/ kg of Gallic acid for 2 weeks. Then, the hippocampal level of
BDNF and TNF- was performed for all groups by Elisa.
Results: The findings of present study showed that the hippocampal level of BDNF in all test
groups are significantly higher than TMT group (p ≤0.05) and the hippocampal level of TNF -a in
all test groups is significantly less than the TMT group (p ≤0.05).
Conclusion: Endurance exercises training, chronic administration of GA and co-treatment with
training and GA consumption specially, having sufficient neurotrophic and immune modulator
effects in male rats with Alzheimer.
Keywords: Alzheimer’s disease, Endurance training, Gallic acid, BDNF, TNF-a, hippocampus