*Corresponding Author: Pusapati Madan Ranjit, Email: madanranjit@gmail.com ISSN 0976 – 3333 ORIGINAL RESEARCH ARTICLE Available Online at www.ijpba.info International Journal of Pharmaceutical & Biological Archives 2013; 4(1): 235-239 Antimicrobial and Antihelminthic Activities of Various Extracts of Leaves and Stems of Abutilon indicum (Linn.) Pusapati Madan Ranjit 1 *, Y. Ankamma Chowdary 1 , Harika Krapa 1 , Sindhu Nanduri 1 , Himabindhu Badapati 1 ,, Kola Phani Kumar 2 , Priyanka Bommadevara 1 , Madhuri Kasala 1 1 NRI College of Pharmacy, Pothavarappadu (V), Agiripalli Mandal, Krishna (Dist.), A.P, India 2 Guntur (Dist.), A.P, India ANU College of Pharmaceutical Sciences, Nagarjuna Nagar, Nagarjuna University, Received 28 Oct 2012; Revised 07 Feb 2013; Accepted 18 Feb 2013 ABSTRACT Our present study carried out the antimicrobial and antihelminthic activity of leaves and stems of the plant Abutilon indicum (Linn.) Sweet (Malvaceae).Different parts of the plant are used for treatment of various ailments in ethnomedicine. We are carried out the antimicrobial activity chloroform extract prepared form leaves using agar-well diffusion method against both gram positive and gram negative microorganisms and antihelminthic activity was performed by using alcoholic extract of stems, ethyl acetate and carbon tetra chloride fraction of aqueous extracts from the leaves of plant against Pheretima posthuma. Key words: Antimicrobial activity, Antihelminthic activity, ethnomedicine, Abutilon indicum, Pheretima posthuma. INTRODUCTION Abutilon indicum (Linn.) Sweet (Malvaceae) is a shrub distributed throughout India and other topical regions of the world. The various parts of the plant (leaves, roots, seeds and seed and seed oil) are widely used in variety of ailments in traditional system of medicine such as Ayurveda and Siddha. The roots are useful in treating uterine hemorrhagic discharges. Leaves are useful in treatment toothache, lumbago, piles and all kinds of inflammation. Bark is used as anthelmentic and diuretic [1] . In addition it also showed antispasmodic, cardiac depressant, estrogenic, antifungal and hepatoprotective properties [2-5] . Dried whole plant was used as laxative and demulcent [6] , febrifuge, anthelmintic, diuretic, anti–inflammatory, especially in uterine discharges, piles, lumbago [7] anti inflammatory, immuno stimulant, piles and gonorrhea treatment. Roots and bark are used as aphrodisiac, anti diabetic, nervine tonic, and diuretic. Seeds also used as aphrodisiac, in treatment of urinary disorders [8] . The whole plant is reported to have analgesic [9] , hypoglycemic [10] , hepato protective [11] , hyperlipidemic activity [12] . The various leaves extract was reported to contain alkaloids, flavonoids, sterols, triterpenoids, and glycosides [13-15] . The other phytochemical constituents present in this plant are include fatty acids, abutilin A, flavonoids, quercetin, glycosides, alkaloids, steroids, terpenoids, saponins, sesquiterpenes, lactones, gallic acid, β-sitosterol, geranoil, caryophylline and phenolic compounds [16 -18] MATERIALS AND METHODS . The aim of the present research is, to determine the preliminary phytochemical constituents, antimicrobial and antihelmenthtic activity of various extracts of the leaves and stems of Abutilon indicum. Collection of plant material: Abutilon indicum (linn.) leaves and stem are collected in the month of July and August from the area Pothavarappadu (V), Agiripalli (M), Krishna District, Vijayawada. The collected parts of the plant materials were authenticated by Dr. K. Madhava Chetty, Asst. Professor, Dept. of Botany, Sri Venkateswara University, Tirupati (DRM/NRI/15/08/2011/AC2). Herbarium specimen was deposited in the Department of Pharmacognosy, NRI College of Pharmacy. (Specimen no.NRI/COL/P.COG/AILS).