International Engineering, Science and Education Conference, December 2016 184 COMPARISON OF NUMERICAL TAXONOMY AND 16S rRNA SEQUENCING OF Anoxybacillus pushchinensis (KB4) Fatma MATPAN BEKLER 1* , 1 Dicl fmatpan@dicle.edu.tr 2 2 syalaz@dicle.edu.tr ABSTRACT: Species of Anoxybacillus are widespread in geothermal springs, manure, and milkprocessing plants. Recently, isolation and identification of Anoxybacillus have gained more importance because of there are many fields in biotechnology (enzyme production, bioremediation, secondary metabolites, renewable energy, removing heavy metals). Formerly, Anoxybacillus species has known as Bacillus species, but firstly it has been recognized as Anoxybacillus in 2000 in the literature. There are some deficiencies about identification of this species. Morphological, physio-biochemical tests and 16S rRNA analyzes may be insufficient alone, when doing idenfication of bacteria. 16S rRNA sequence analysis results should be used with standard identification methods and especially new species should be reported carefully. In this study, Anoxybacillus pushchinensis (KB4), which is isolated, determined phenetics, analyzed with 16S rRNA sequences before, was used for numerical taxonomy and it was compared with molecular identification. The UPGMA method was used for numerical taxonomy and the phenogram was drawn with MATLAB. According to the numerical definition result and 16S rRNA analysis, KB4 strain was highly similar to Anoxybacillus pushchinensis DSM 12423 T . Results showed that reliability of this research is considerably higher than previous assumption. Key words: Anoxybacillus, numerical taxonomy, 16S rRNA, UPGMA. Anoxybacillus pushchinensis (KB4) 16S rRNA SEKANSLAMASI VE Anoxybacillus iyoremediasyon, sekonder metabolit Anoxybacillus Bacillus Anoxybacillus - biyokimyasal testler ve 16S rRNA analizleri yeterli olmayabilir. Anoxybacillus pushchinensis (KB4) 16 Anoxybacillus pushchinensis DSM 12423 T b nin ndan Anoxybacillus, 16S rRNA, UPGMA.