13 Inhibition of Virulence Potential of Vibrio Cholerae by Herbal Compounds Sinosh Skariyachan Department of Biotechnology Engg, Dayananda Sagar Institutions, Bangalore, India Email: sinoshskariya@gmail.com 13.1 Introduction Vibrio cholerae is notorious pathogenic bacteria responsible for enormous public health burden, especially in developing countries all over the world. Most common impact of this human pathogen is cholera, a self-limiting illness. The outbreak of cholera is responsible for approximately 120, 000 deaths every year and has a major impact on the health of young children between the ages of 1 and 5 years [1]. At present, the treatments against cholera and related outbreaks are very critical issue worldwide as most of the strains have developed multiple drug resistance to all classes of conventional chemother- apeutic agents. These circumstances underline the necessity for alternative and promising strategies and development of novel therapeutic solutions. The present chapter is mainly focused on the utility of natural remedies against Vibrio cholerae infection when all conventional drugs seem to have failed. Initial section of the chapter emphasizes the basic microbiology, pathogenicity, virulent factors and their mode of action for Vibrio cholerae.Later sections explain the current global scenario of the evolution of multidrug resistant (MDR) strains and development of alternative natural remedies against MDR strains of Vibrio cholerae. 13.2 Microbiology of Vibrio Cheolrae Vibrio cholerae is one of the most notorious pathogenic bacterium and belongs to the Vibrionaceae family. It is Gram-negative, aerobic or facultatively anaerobic, non-sporulating, curved rod shaped organism of 1.4–2.6 mm 337