Volume 3 • Issue 1 • 1000116
J Tumor Res, an open access journal
ISSN: JTR
Research Article Open Access
Montero et al., J Tumor Res 2017, 3:1
DOI: 10.4172/jtr.1000110
Research Article OMICS International
Journal of Tumor Research
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*Corresponding author: Nieto-Villar JM, Department of Chemical-Physics, A.
Alzola Group of Thermodynamics of Complex Systems M.V. Lomonosov, University
of Havana, Havana, 10400-Cuba, Tel: +351 220428557; E-mail: nieto@fq.uh.cu
Received February 07, 2017; Accepted February 24, 2017; Published February
28, 2017
Citation: Montero S, Durán I, Pomuceno JP, Martín RR, Mesa MD, et al. (2017)
How much Damage can make the Glucose in Cancer? J Tumor Res 3: 116.
Copyright: © 2017 Montero S, et al. This is an open-access article distributed
under the terms of the Creative Commons Attribution License, which permits
unrestricted use, distribution, and reproduction in any medium, provided the
original author and source are credited.
How much Damage can make the Glucose in Cancer?
Montero S
1,2
, Durán I
1
, Pomuceno JP
1
, Martín RR
1
, Mesa MD
1
, Mansilla R
3,1
, Cocho G
4,1
and Nieto-Villar JM
1
*
1
Department of Chemical-Physics, A. Alzola Group of Thermodynamics of Complex Systems M.V. Lomonosov, University of Havana, Havana, Cuba-10400
2
Departement of Basics Science, University of Medical Science of Havana, Havana, Cuba-10400
3
Centro de Investigaciones Interdisciplinarias en Ciencias y Humanidades, UNAM, México
4
Instituto de Sistemas Complejos C3 and Instituto de Física de la UNAM, México
Abstract
Using the entropy production rate method to determine the inluence of glucose on a glycolysis model for HeLa
tumor line, the greater complexity and robustness hypoglycemic phenotype was established. On the other side,
inside the same metabolic phenotype a higher glucose concentration corresponds to a higher entropy production
rate. It is concluded that this behavior is indicative of the directional character and stability of the dynamical behavior
of cancer glycolysis.
Keywords: Cancer; Glycolysis; Entropy production rate; Glucose
Introduction
Cancer is a generic name given to a complex interaction network of
malignant cells that have lost their specialization and control over normal
growth. his network could be modelled as a nonlinear dynamical
system, self-organized in time and space, far from thermodynamic
equilibrium, exhibiting high complexity, robustness, and adaptability
[1]. Cancer cells, for the most part, show a high glycolytic rate and
low pyruvate oxidation rate compared to normal cells, which brings
with it a high consumption of glucose of the extracellular medium
and an increase in the production of lactate, phenomenon known
like “Warburg Efect” [2]. Increased glycolytic rate is beneicial for
cell proliferation, as it provides the intermediates necessary for the
synthesis of new nucleotides, lipids, amino acids and the generation
of reducing power, all of which are necessary for cell division [3].he
molecular mechanism behind the constitutive overexpression of aerobic
glycolysis is not yet fully deined. Activation of oncogenes and tumor
suppressor genes such as ras, c-myc, src and TP53 have implications
for the regulation of aerobic glycolysis. hese genes cause alterations
in signalling pathways of growth factors and these in turn exert control
over cellular metabolism [4]. Due to a combination of high glucose
consumption rates by tumor cells and reduced tumor vascularization,
the glucose concentration in the tumors can be 3 to 10 times lower than
in normal tissues, according to the stage of its development. herefore,
tumor cells must develop strategies for their growth and survival in
metabolically unfavorable environments [5]. Since the last years, cancer
glycolysis has been a target in oncology research [6]. he signiicant
increase of glycolysis rate observed in tumors has been recently veriied,
yet only a few oncologists or cancer researchers understand the full
scope of Warburg’s work [6,7] despite of its great importance. Altered
energy metabolism is proving to be as widespread in cancer cells as
many of the other cancer-associated traits that have been accepted
as hallmarks of cancer [8]. he regulation of metabolism, relevant
to senescence process, would be a key to improve and identify new
anti-cancer therapies in the future. he complex systems theory and
the thermodynamics formalism in the last years have shown to be a
theoretical framework as well as a useful tool to understand and forecast
the evolution of the tumor growth [9-17]. he goal of this work is to
extend the thermodynamics formalism previously developed [18-22] to
the metabolic rate of human cancer cells. he manuscript is organized
as follow: Firstly, a theoretical framework based on thermodynamics
formalism, particularly the entropy production rate is presented. hen
results and discussion are presented. Finally, some concluding remarks
are presented.
Materials and Methods
Kinetic model of cancer glycolysis
he model used was proposed by Marin et al. [23] for the glycolytic
network of HeLa tumor cell-lines growth under three metabolic states:
Hypoglycemia (2.5 mM), Normoglycemia (5 mM) and Hyperglycemya
(25 mM) during enough time to induce phenotypic change in cellular
metabolisms. However, the growth saturation was not attained in this
phase. In the other stage the cells were exposed to diferent glucose
concentrations: 2.5 mM, 5 mM y 25 mM until they reach the stationary
state. he rate of entropy production was calculated using the glycolysis
network model of HeLa cell lines at steady state. he modelling of the
metabolic network was made in the biochemical network simulator
COPASI v 4.6 (Build 32) (http://www.copasi.org). he parameters and
concentration values used were reported by Marin et al. [23].
hermodynamics framework
As we know from classic thermodynamics, if the constraints of
a system are the temperature T and the pressure P, then the entropy
production can be evaluated using
Gibbs’s free energy [24], as:
1
i Tp
S dG
T
δ =−
(1)
If the time derivative of (1) is taken, we have that:
1
Tp
i
dG
S
dt T dt
δ
=−
(2)