Imperial Journal of Interdisciplinary Research (IJIR) Vol-2, Issue-10, 2016 ISSN: 2454-1362, http://www.onlinejournal.in Imperial Journal of Interdisciplinary Research (IJIR) Page 1743 In Vitro AntibiofilmAnd Antibacterial Activity Of HyssopusOfficinalis Extract And Its Oil Against Pseudomonas Aeuroginosa Aseel Mohammed Hamzah Zoonotic Disease Unit / Veterinary Medicine College / Baghdad University – Iraq Abstract :This study aimed to evaluate the antibacterial and antibiofilm activity of Hyssopus officinalis extract and its oil against Pseudomonas aeuroginosa.Hyssopus officinalis oil was obtained using hydro distillation whileHyssopus officinalis extract was obtained by an ethanolic extraction method and both extracts submitted to the antimicrobial assay by disc diffusion method and tube broth dilution assays (minimum inhibitory (MIC) and bactericidal (MBC) concentrations) against Pseudomonas aeuroginosa. Biofilms formed were examined using tube assay . The results exhibited significant activity against Pseudomonas aeuroginosabacteria with inhibition zone and minimal inhibitory concentration value in the range of 7–16 mm and 0.5–1 μl/ml, respectively. Key words: Hyssopus officinalis extract, Hyssopusofficinalisoil,Pseudomonas uroginosa,antibiofilm,antibacterial Introduction In the last century the search for antimicrobial drugs from medical herbals and their essential oils has been of great interest, the infectious diseases are known to have been treated with texture derived from plants, however, antibiotic resistance has growing ultimately in the recent years and is discomposing an ever rising therapeutic trouble(1). Pseudomonas aeruginosa is a periodic causative agent in healthcare associated infections (2) furthermore P. aeruginosa is the most common Gram-negative pathogen causing nosocomial pneumonia in Iraqi hospitals (3,4), and have beenassign to contamination of inanimate objects in the hospitalsetting and simplify by healthcare workers who may carry thispathogen via direct person-to-person contact Today, fullyactive antibiotic options available to treat nosocomial infectionsdue to multidrug-resistant (MDR) P. aeruginosa are extremelylimited (5,6), Furthermore, diverse resistance mechanisms were found in these MDR isolates. Bacteria more resistant to manyantimicrobial agents when itprovides a biofilmon a surface than the same bacteria growing in afree - swimming state(7). The active ingredient isolated frommedicinal herbs have been studied for theirantibacterial effects against planktonic bacteria. Furthermore, some plants have been studied their ability to prevent the formation of biofilm in some pathogens such as Listeria monocytogenes (8) and Pseudomonas aeruginosa (9). The aims of this study were to evaluate the antibacterial activity of Hyssopus officinalis plant extracts (crude and oil) as the minimal inhibitory concentration (MIC) by diffusion and dilution methods for P. aeruginosa and to study the antibiofilm activity of Hyssopus officinalis against P. aeruginosa . Material and method 1. Preparation of Hyssopus officinalis extracts The plants were purchased from markets in Baghdad.Grinding the plant and the powdered plant material (250 g) was extracted in a 1000 ml conical flask with 500ml solvents(ehanol,w:v ,1:2) for 9 days in freeze after that filtered using Whattman No 4 filter paper. The filtrate obtained was concentrated by evaporated to dryness to obtain the crude extract. The crude extracts were kept at 4°C until further uses. 2.Preparation of Hyssopus officinalis oil by Steam distillation Thirty gram of the plant put in a pressure cooker and add water about three litter then closed the pressure cooker and we put the Gasoline transfer hose on Slot steam out fixed it with dough until dry, after that closed the pressure cookerand put cooker on fire then put the rest of Gasoline transfer hose on acontainor fulled with water to allow condence the steam in Gasoline transfer hose and collected the oil at the end of hose in sterile bottle glass, the crude extracts were kept at 4°C until further uses.