1 MCR-14-0420 Revision Version 2 RhoC is an Unexpected Target of RhoGDI2 in Prevention of Lung Colonization of Bladder Cancer Erin M. Griner 1 , Garrett M. Dancik 2 , James C. Costello 3 , Charles Owens 3 , Sunny Guin 3 , Michael G. Edwards 4 , David L. Brautigan 1 , Dan Theodorescu 3,5 1 Center for Cell Signaling and Department of Microbiology, Immunology and Cancer Biology University of Virginia, Charlottesville, VA, USA, 22908 2 Department of Mathematics and Computer Science, Eastern Connecticut State University, Willimantic, Connecticut, 06226 3 Department of Surgery and Pharmacology and 4 Division of Pulmonary Sciences and Critical Care Medicine, University of Colorado, Aurora, Colorado, USA, 80045 5 University of Colorado Comprehensive Cancer Center, Aurora, Colorado, USA, 80045 Running Title: RhoC is negatively regulated by RhoGDI2 Keywords: Guanine Nucleotide Dissociation Inhibitors, Rho GDP dissociation inhibitor 2, RhoC, lung colonization, metastasis Financial Support: Supported by National Institutes of Health grant CA143971 to DT, CA910935 to support E.M.G. and the Paul Mellon Urological Cancer Research Institute at the University of Virginia. Corresponding author: Dan Theodorescu, Departments of Surgery and Pharmacology and University of Colorado Comprehensive Cancer Center, Aurora, CO 80045, Tel: 303-724-7135, Fax: 303-724-3162, E-Mail: dan.theodorescu@ucdenver.edu. Conflict of Interest: The authors declare no conflict of interest. Word Count: 5,169 Total number of Figures: 6 on December 18, 2014. © 2014 American Association for Cancer Research. mcr.aacrjournals.org Downloaded from Author manuscripts have been peer reviewed and accepted for publication but have not yet been edited. Author Manuscript Published OnlineFirst on December 16, 2014; DOI: 10.1158/1541-7786.MCR-14-0420