International Journal of Sciences: Basic and Applied Research (IJSBAR) ISSN 2307-4531 (Print & Online) http://gssrr.org/index.php?journal=JournalOfBasicAndApplied --------------------------------------------------------------------------------------------------------------------------- ATP-Induced Nuclear and Cytosolic Ca 2+ Signal in Various Stages of Interphase HeLa/Fucci2 Cell Gabriel J. Mchonde a *, Yoh-ichi Satoh b , Tomoyuki Saino c a,b,c Department of Anatomy and Cell Biology, School of Medicine, Iwate Medical University, Iwate Prefecture, Japan. a Department of Biomedical Sciences, School of Medicine and Dentistry, The University of Dodoma, Dodoma, Tanzania a Email: gmchonde@yahoo.co.uk or mchonde@iwate-med.ac.jp b Email: yisatoh@iwate-med.ac.jp c Email: tsaino@iwate-med.ac.jp Abstract Cell cycle progression involves the coordination of growth, DNA replication and division in proliferating cells. These intracellular activities are linked with calcium signaling in which calcium ion (Ca 2+ ) act as a second messenger or as an agent for phosphorylation processes. Previous studies have examined the Ca 2+ in the dividing cells; however, none of these gave the detailed explanation on Ca 2+ dynamics in an interphase cell. This study addresses here the issue of whether the nuclear and cytosolic Ca 2+ concentrations ([Ca 2+ ] n and [Ca 2+ ] c ) changes are related or not in the dividing interphase cells. Current approach used interphase HeLa/Fucci2 cells to investigate the intracellular Ca 2+ dynamics between the nucleoplasm and cytoplasm following stimulation with extracellular adenosine triphosphate (extracellular purinergic-2 receptor agonists). The results revealed that [Ca 2+ ] n and [Ca 2+ ] c signals differed both between and within interphase stages. ------------------------------------------------------------------------ * Corresponding author. E-mail address: gmchonde@yahoo.co.uk or mchonde@iwate-med.ac.jp 274