CHAPTER 8
MolecularImaging:Physicsand
BioapplicationsofQuantumDots
Xavier Michalet, Laurent A. Bentolila, and Shimon Weiss
111
8.1 Introduction 112
8.2 Brief Overview of Quantum Dot Physics 112
8.2.1 Conduction and Valence Bands 112
8.2.2 Quantum Confinement 113
8.2.3 Electronic Structure 114
8.3 Optical Properties of Quantum Dots 115
8.3.1 Absorption and Emission Spectra 115
8.3.2 Fluorescence Intermittency 115
8.3.3 Spectral Diffusion 117
8.3.4 Polarization 117
8.3.5 Fluorescence Lifetime and Antibunching 117
8.3.6 Lasers, LED, and Photovoltaic Cells 117
8.4 Synthesis of Colloidal Nanocrystals 119
8.4.1 Synthesis 119
8.4.2 Solubilization 120
8.4.3 Functionalization 121
8.5 Applications of Quantum Dot in Biological Imaging 121
8.5.1 Immuno-Cytochemistry and Fluorescence in situ Hybridization 122
8.5.2 Live Cell Imaging 123
8.5.3 Molecular Imaging 124
8.6 Conclusion 124
8.7 References 125