World Journal of Fish and Marine Sciences 7 (4): 325-333, 2015
ISSN 2078-4589
© IDOSI Publications, 2015
DOI: 10.5829/idosi.wjfms.2015.7.4.96230
Corresponding Author: Amira A.Z. Omer, Department of Fish Diseases and Management, Faculty of Veterinary Medicine,
Kafr El-Sheikh University, Egypt. Tel: +01020517904, E-mail: amira_vet2007@yahoo.com.
325
Role of Some Herbs in Controlling Aflatoxicosis in Cultured Oreochromis niloticus
Mohamed M.A. Zayed, Riad H. Khalil, Talaat T. Saad and Amira A.Z. Omer
1 2 2 3
Department of Fish Diseases, Animal Health Research Institute Kafr El-Sheikh Branch, Egypt
1
Department of Poultry and Fish Disease, Faculty of Veterinary Medicine, Alexandria University, Egypt
2
Department of Fish Diseases and Management,
3
Faculty of Veterinary Medicine, Kafr El-Sheikh University, Egypt
Abstract: The present study provides that the black seed is the best herbs that decrease the aflatoxin effect in
cultured Oreochromis niloticus followed by garlic and onion, respectively. The results showed that level of
RBCs and WBCs, differential leucocytic counts and phagocytosis process improved with addition of black
seed, garlic and onion. The residue of aflatoxin in fish flesh decreased in the groups treated with black seed,
garlic and onion than the control or fish fed on the aflatoxin. The results proved that, frequent supplementation
of fish ration with black seed, garlic and onion can reduce the aflatoxin hazards in the fish. Also the economic
efficiency measures (total return, total costs, net profit, total returns/total costs and net return to total costs)
improved in the groups fed with black seed, garlic and onion and when all of them added to the fish treated with
aflatoxin diet improved economic efficiency results than the group treated with aflatoxin only.
Key words: Economic Efficiency Black Seed Aflatoxin
INTRODUCTION aflatoxin. Both Ginseng extract and Nigella sativa Oil 1%
The using of synthetic drugs in the therapeutic field Nigella sativa showed more improvement of all enzymes
may have adverse effects which may be more dangerous of kidney and liver and also total lipid and cholesterol
than the disease itself by Fluk et al. [1], from this fact and were reduced and body weight increased with
with the call of “Back to nature” medical plants consider improvement of economic and productive efficiency of
an important target area for several studies to show fish production farms. The green onion has antifungal
possibility of its use in a variety of health problems. activity on A. parasiticus that producing aflatoxin and has
The garlic has antiviral, antibacterial, antifungal and carceingenic effect on the human, according to Kim [3]. So
antiparasitic effect. Garlic is nicknamed Russian penicillin this study was carried-out to throw the light on using the
for its widespread use as a topical and systemic natural feeds on the improving immunity of the fish
antimicrobial agent. Allicin has antimicrobial effects Oreochromis niloticus and its role in the prevention of
in vitro against many viruses, bacteria, fungi and Aflatoxicosis in the fish through study the effect of black
parasites, but dried, powdered and oil preparations of seed (Nagilla sativa), garlic and onion on the number of
garlic have not been shown to have significant RBCs, WBCs and its differentials, Phagocytic activity and
antimicrobial activity. Also, it has antifungal effect, thus phagocytic index as well as their effect on the residues of
Garlic enjoys a worldwide reputation as an antifungal the Aflatoxin in fish flesh.
folk remedy. Mona et al. [2], reported that, Aflatoxin
the major toxic metabolites of fungi which are able to MATERIALS AND METHODS
induce chronic liver damages. The antioxidant and
hepatoprotective effects of Ginseng extract and Nigella A total number of 240 healthy Nile Tilapia
sativa Oil 1% on Aflatoxin was investigated. Moreover (Oreochromis niloticus) were collected from Barseek fish
the liver exhibited some clinico-pathological changes and farm at Behera Governorate. Fish were transported alive
decreased body weight due to the toxic effects of to the laboratory in plastic bags containing water
reduced the development of hepatotoxicity by Aflatoxin.