Volume 4 • Issue 5 • 1000144
J Develop Drugs
ISSN: 2329-6631 JDD an open access journal
Research Article Open Access
Journal of Developing Drugs
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ISSN: 2329-6631
Hamza et al., J Develop Drugs 2015, 4:5
http://dx.doi.org/10.4172/2329-6631.1000144
Keywords: α-Glucosidase inhibitors; Alternative drugs; Flavonoids;
Polyphenols
Introduction
Diabetes, a multifactorial disease afecting over 347 million people
worldwide, is characterized by hyperglycemia resulting from defects in
insulin secretion, insulin action, or both [1]. Diabetes not only kills, but
is a major cause of adult blindness, kidney failure, gangrene, neuropathy,
heart attacks, and strokes. here has been a striking emergence of non-
insulin-dependent diabetes mellitus (type 2 diabetes) as a major health
problem in populations undergoing modernization of life-style, both in
developing nations and in developed countries. Over 90% of patients
with diabetes have type 2 diabetes, while the remaining has type 1
diabetes [1].
α-Glucosidase inhibitors are a class of drugs used to treat non-
insulin dependent diabetes by inhibiting α-glucosidase (EC 3.2.1.20,
α-D-glucoside glucohydrolase), a membrane-bound intestinal enzyme
that catalyze the hydrolysis of the α -glycosidic bond of oligosaccharides
to liberate glucose. Liberated glucose is absorbed from the intestine and
contributes to postprandial hyperglycemia. α-Glucosidase inhibitors
prevent or delay the hydrolysis or absorption of carbohydrates and
reduce post prandial hyperglycemia, making such inhibitors useful in
the management of type 2 diabetes [2]. Currently, acarbose, voglibose
and miglitol are three such α -glucosidase inhibitors used clinically
to control blood glucose levels of patients, although they can cause
negative gastrointestinal symptoms [3,4].
Published surveys indicated that more than 1200 plants are used
worldwide in traditional medicine for their alleged hypoglycemic
activity [5,6]. A study on traditional herbal remedies for diabetes may
provide valuable leads for the development of alternative drugs and
therapeutic strategies [6]. he popular hypoglycemic drug glucophage
(metformin), for instance, is derived from Galega oicinalis [7] (Table
1).
Since polyphenols possess multiple biological activities and
constitute an important part of the human diet, they have recently
emerged as critical phytochemicals in type 2 diabetes prevention and
treatment. heir hypoglycemic action results from their antioxidative
efect involved in recovering of altered antioxidant defenses and
restoring insulin secreting machinery in pancreatic cells, or abilities to
inhibit the activity of carbohydrates hydrolyzing enzymes (α-amylase
and α-glucosidase) [4].
In addition, some lavonoids and polyphenols as well as
sugar derivatives (acarbose) are found to be efective in inhibiting
α-glucosidase [4,8-12]. herefore, discovery of α-glucosidase inhibitors
from traditional plants is very helpful in the development of new anti-
diabetic drugs.
he objective of this research was to investigate the α-glucosidase
inhibitory activity of the most widely anti-diabetic plants that used
traditionally in Asia and Mediterranean regions for treatment of
diabetes. Most of medicinal herbs evaluated in this study are used
traditionally as anti- diabetic plants and were selected here on the
basis of their use in traditional medicines throughout Asia and
Mediterranean regions moreover they previously were found to induce
hypoglycemic activity in experimental animals. hese plants are shown
in Table 2. Extracts of these plants were tested for their inhibition
of α -glucosidase. Moreover, the relationships between phenolic
compounds and biological efects were investigated.
Materials and Methods
Materials
Dry samples of selected plants were purchased in a local market,
Al Ain, UAE. he authentications of the plants were carried out at the
Herbarium of the Department of Biology, UAE University, Al Ain,
Abstract
Inhibition of α-glucosiase and the associated reduction of glucose absorption is an attractive approach for decreasing postprandial
hyperglycemia and for the discovery of potent antidiabetic agents. One of the most important sources of potential α-glucosiase inhibitors
represents the class of polyphenols. This paper aims to evaluate previous herbal polyphenol-rich extracts plan on the management
of diabetes mellitus, to address their α-glucosidase inhibitory activity. Polyphenol-rich extracts from thirteen widely used traditionally
anti-diabetic plants in Asia and Mediterranean regions were evaluated for their potential α-glucosidase inhibitory activity. Among these
evaluated plants, 10 were much stronger than that of acarbose standard. Punica granatum manifested the highest inhibitory activity with
IC
50
at 3.59 ± 0.11 µg/mL, followed by Psidium guajava with IC
50
at 8.08 ± 0.10 µg/mL and Cinnamomum zeylanicum with IC
50
at 9.87.08
± 0.14 µg/mLA. A high correlation (r=0.65, p<0.001) was observed between α-glucosidase inhibition and total phenolic content of all
plants. Punica granatum, P. guajava, C. zeylanicum and Ziziphus spina-christi had also the highest total phenolic content. Extracts for
the above studied plant species may potentially replace acarbose in its current clinical use in improving post-prandial glycaemic control
in type 2 diabetics. As a result, these polyphenol-rich extracts potentially offer a complementary approach to develop functional food and
potential antidiabetic agents.
*Corresponding author: Alaaeldin A. Hamza, Hormone Evaluation Department,
National Organization for Drug Control and Research (NODCAR), Giza, Egypt, Tel:
20-01201991433; E-mail: alaa17mm@gmail.com
Received November 16, 2015; Accepted November 30, 2015; Published
December 07, 2015
Citation: Hamza AA, Ksiksi TS, Shamsi OAAl, Balfaqh SA (2015) α-Glucosidase
Inhibitory Activity of Common Traditional Medicinal Plants Used for Diabetes Mellitus. J
Develop Drugs 4: 144. doi:10.4172/2329-6631.1000144
Copyright: © 2015 Hamza AA, et al. This is an open-access article distributed
under the terms of the Creative Commons Attribution License, which permits un-
restricted use, distribution, and reproduction in any medium, provided the original
author and source are credited.
α-Glucosidase Inhibitory Activity of Common Traditional Medicinal Plants
Used for Diabetes Mellitus
Alaaeldin Ahmed Hamza
1
*, Taouik Saleh Ksiksi
2
, Obaid Ali Al Shamsi
2
and Salem Abobakr Balfaqh
2
1
Hormone Evaluation Department, National Organization for Drug Control and Research (NODCAR), Giza, Egypt
2
Department of Biology, Faculty of Science, UAE University, Al Ain, UAE