84 South Indian Journal of Biological Sciences 2015; 1(2); 84-89 Online ISSN:2454-4787 Genotoxic efects of GSTP1 gene polymorphisms with respect to respiratory problems among traic police Murugesan Mahendran 1, *, Pardhanandana Reddy 2 , Rajagopal Raskin Erusan 3 , Marimuthu Ramar 4 , Pandian Manonmani 5 1 Institute of Genetics & Hospital for Genetic Diseases, Osmania University, Hyderabad, India 2 Institute of Genetics & Hospital for Genetic Diseases, Osmania University, Hyderabad, India 3 Department of Genetics, Dr.A.L.M. Post Graduate Institute of Basic Medical, Sciences, University of Madras, Chennai-600113, Tamil Nadu, India. 4 Division of Entomology, Department of Zoology, School of Life Sciences, Bharathiar University, Coimbatore, Tamil Nadu 641046, India 5 Department of Biotechnology, Centre for Research and Development, PRIST University, Vallam, hanjavur *Corresponding Author: MurugesanMahendran Manuscript details Abstract Article History: Received 15 July 2015 Revised 14 August 2015 Accepted 20 September 2015 Published 29 September 2015 Keywords: GSTP1, Gene polymorphism, Respiratory problems, Traic policemen. Genetic susceptibility is likely to play a role in response to vehicular pollution of GSTP1 and NAT2 (C481T). Genotoxic efects due to respiratory problems in traic police occupationally exposed to vehicular exhaust have particular impor- tance in the detoxiication of inhaled toxicants since it is the most abundant in the lung function and the association.161 male traic policemen and 170 con- trol subjects were selected for this study. Gene polymorphism was studied using PCR and RFLP method. he results on gene polymorphism of GSTP1 and NAT2 (C481T) showed signiicant association of homozygous mutant genotype (GG) in GSTP1 gene and homozygous mutant genotype (CC) in NAT2 gene with traic police having respiratory problems. However, no such association was observed in traic police without respiratory problems and control group also. Our results suggest that long term exposure to urban air pollution (with traic as the main contributor) polymorphisms in genetic susceptibility provides evidence to sug- gest a role in determining the respiratory problems in GSTP1 and NAT2 (C481T) variants associated with reduced detoxiication ability, increase susceptibility to such damage and appears to be associated with occupationally exhaust vehicular exhaust in traic policemen. 1. Introduction Polymorphisms in genes of xenobiotic metabolizing enzymes are expected to modulate individual re- sponses to carcinogens (Vineis 2002; Mehrotra et al., 2010; Kukkonen et al., 2011). Incorporation of such susceptibility markers in polymorphisms studies may improve the precision of carcinogen exposure and health risk estimates (Perera and Weinstein 2002; Kamel and Hoppin 2004; Ryan et al., 2007). GSTP1 variant proteins that are thought to function in xe- nobiotic metabolism and play a role in susceptibili- ty of diseases (Spiteri et al., 2000; Poloninkov et al., 2009). Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles and involve the detoxiication of envi- ronmental pollutants (Bi et al., 2007; Hu et al., 1999). he highly homologous human gene N-acetyltrans- ferase (NAT2), and appear to code for the genetically invariant and variant NAT proteins, respectively. Be- cause they catalyze the N-acetylation (usually deacti- vation) and O-acetylation (usually activation) of ar- omatic and heterocyclic amine carcinogens (Hein et al., 2000; Hong et al., 2007). Genetic polymorphisms NAT2 may modify risk associated with carcinogen exposures because of their high prevalence in the general population, genetic variants that determine