International Research Journal of Agricultural Science and Soil Science Vol. 1(3) pp.098-108, May 2011
Available online@ http://www.interesjournals.org/IRJAS
Copyright © 2011 International Research Journals
Full Length Research Paper
Impact of dual inoculation with Rhizobium and
Trichoderma on damping off, root rot diseases and
plant growth parameters of some legumes field crop
under greenhouse conditions
W. I. Shaban
1
and M. A. El-Bramawy
2*
1
Agricultural Botany Department and
2
Agronomy Department, Faculty of Agriculture, Suez Canal University, 41522
Ismailia, Egypt
Accepted 11 February, 2011
Damping off and root rot are a worldwide diseases, caused destructive yield losses of legumes field
crop of Vicia fabae, Cicer arietinum and Lupines terms. The biological control of damping off and root
rot diseases using microorganisms antagonistic to growth and development of the fungi were
investigated under greenhouse conditions at the Experimental Farm, Faculty of Agriculture, Suez
Canal University, Ismailia, Egypt. The results exhibited that Rhizobium spp. and Trichoderma sp. fungi
scoffed at the potential or ability to their compatibility and in combined effects in controlling the fungi,
which caused the damping off and root rot. This resulted in an improvement in many plant growth
parameters, which are ultimately expressed in increasing the yield components of branches plant
-1
,
pods plant
-1
, seeds pod
-1
, mean seed weight and then increase seed yield of the legume field crops
broad bean, chickpea and lupine plants. The results suggested that the Rhizobium spp. and
Trichoderma sp. can be used as biological control of some soil-borne fungal diseases causing
significant yield losses in legumes field crops.
Keywords: Soil borne diseases, Faba bean, Chickpea, Lupine, yield characteristics
INTRODUCTION
Faba bean (Vicia faba L.), Chickpea (Cicer arietinum L.)
and lupine (Lupines terms L.) are the most important
legumes crops in the human feeding all over the world.
Grain legumes are subject to numerous pathogens
attacking both the roots and the aerial parts of the plant.
Among biotic stresses, diseases are often the major
limiting factor the yield of grain legumes, and they are
economically important where these crops are grown
(Puglia and Aragona, 1997). Soil-borne fungal diseases
are the most important factors among all limiting the seed
yield of legume crops in many countries worldwide.
On the other hand, regarding to environmental and
health concerns about the extended use of effective
chemicals, which are available to control a number of
*Corresponding author Email: el_bramawy71@hotmail.com
these diseases, we cannot rely upon them to a large
extent for both economical and environmental reasons.
The best approach is searching about a considerable
interest in finding alternative control approaches for use
in biological control strategies for crop diseases
(Raupach and Kloepper, 1998; Pal and Garedener,
2006). The beneficial effect of Rhizobium sp. has been a
main focus in terms of biological nitrogen fixation in the
recent past (Deshwal et al., 2003). Other reports via,
Nautiyal (1997), Estevez de Jensen et al. (2002) ; Baraka
et al. (2009) revealed that Rhizobium spp. can be used in
control soil-borne pathogens of legume crops, such as
Rhizoctonia spp., Fusarium spp., sclerotium rolfsii,
Macrophomina phaseolina and Pythium spp. As an
extension to talk in this area, biological control of plant
diseases, especially soil-borne plant pathogens, has
been the subject of extensive research in the last two
decades. Trichoderma spp. is well documented as