Pritt Verma et al./ Elixir Appl. Botany 87 (2015) 35578-35583 35578 Introduction The liver is that the largest organ of the body and its management many very important performs in regulation of physiological state, secretion, storage and metabolism by eating substances like lipids, carbohydrates, proteins, coagulation (Rajesh et.al 2004). Liver diseases are the most serious problem and mainly caused by toxic chemicals like, excess consumption of alcohol, high doses of paracetamol etc. within 24 h of ingestion (Maheswari et.al 2008). In the absence of a reliable liver protective drug in the modern system of medicine, a number of medicinal preparations in Ayurveda, the Indian system of medicine, are recommended for the treatment of liver disorders (Chatterjee, 2000). Solanum xanthocarpum (Family Solanaceae) generally known as Yellow Berried Nightshade (syn: Kantakari), is a prickly diffuse bright green perennial herb, woody at the base, 2- 3 m height constitutes all over India in Nov-Jan, mostly in dry places as a weed on roadsides and waste lands (Singh et.al., 2010). This plant is also known as Choti Katheri, Bhutkatya or Bhumiringani (Bhatt et.al., 2011).The whole plant part (leave, fruit, root, flower, stem) reported several type of activity and the part of plant have many chemical constituent life fruits are contain several steroidal alkaloids like solanacarpine (Gupta et.al., 1938) solanacarpidine, solancarpine, solasonine (Saived 1963) and solamargine (Siddiqui et.al., 1983) and the other constituents caffeic acid, coumarins like aesculetin and aesculin, steroids carpesterol, diosgenin, campesterol, daucosterol and triterpenes like cycloartanol and cycloartenol (Paul et.al., 2008). Solanum xanthocarpum has been reported as an effective medicine in the treatment larvicidal (Bansal,2009) anthelmintic (Gunaselvi et.al., 2010) asthma (Savithramma,2007) immunomodulatory (Sultana,2011) antinociceptive (Rahman,2003) antifungal (Saini,2006) larvicidal defect (Mohan et.al.,2007) hypoglycemic (Kar et.al., 2006) anti-inflammatory, analgesic (Thabrew et.al.,2003) antibacterial (Usman et.al., 2010) antimicrobial (Salar et.al.,2009) and wound healing activities (Kumar et.al., 2010). Leaf paste prepared with water is applied topically on forehead to getting relief from had to get relief from headache it has also used in astringent, aphrodisiacs, laxative, diuretic, stomachic, cardiotonic and refrigerant activities (Duraipandiyan et.al.,2006). The stem, flowers and fruits are prescribed for relief in burning sensation in the feet accompanied by vesicular eruptions (Chopra 1956). Choice of scientific and standardized approach to the biological analysis of plant product supported their use within the ancient systems of medication forms the idea for a perfect approach within the development of latest drugs from plants. The current study mainly focused on the liable benefit of hepatoprotective activity of 50% ethonolic whole plant extract SX and also specifies a viable mechanism for its hepatoprotection by analysis with biochemical and histopathological parameter against drug induced hepatotoxicity in rats. ARTICLE INFO Article history: Received: 26 August 2015; Received in revised form: 25 September 2015; Accepted: 01 October 2015; Keywords Solanum xanthocarpum, RIF+INH, SGOT, SGPT, ALP, Total billirubin. Elixir Appl. Botany 87 (2015) 35578-35583 Applied Botany Available online at www.elixirpublishers.com (Elixir International Journal) ABSTRACT The scientific assessment of medicinal plants used in the preparation of folk remedies has contributed modern medicine with effective pharmaceuticals for the treatment of diseases. Objective. The 50% ethonolic extract of solanum xanthocarpum (Solanaceae) whole plant was explored for its hepatoprotective and antioxidant effects on RIF+INH (50 mg/kg) induced acute liver damage in Wistar rats. The Whole plant (SX) were further subjected to various phytochemical study and the studies conclude the presence of alkaloids, flavanoids, glycosides, protein & amino acid etc. Hepatoprotection activity was measured by using enzymatic (serum glutamate oxalate transaminase and serum Glutamate Pyruvate Transaminase (SGOT and SGPT), alkaline phosphatase (ALP), total bilirubin and non enzymatic parameters (GSH, LPO, SOD, CAT) produced significantly increased and decreases serum level in a dose dependant manner. The Whole plant extract SX at the doses of (125 mg/kg &250 mg/Kg) significant liver protective effect by decreasing the serum enzymatic and non enzymatic parameters, although Histopathological profile of liver at dose level 125 mg/kg showing hepatic cells with well preserved cytoplasm prominent nucleus, some of central vein and sinusoids exhibited congestion. At the same time fourth group test dose at 250 mg/kg showing well brought out central vein, hepatic cell with well preserved cytoplasm prominent nucleus. These all result recommended that 50% ethonolic extract of whole plant of Solanum xanthocarpum posses significant hepatoprotective activity. © 2015 Elixir All rights reserved. Assessment of Hepatoprotective Potential of Solanum xanthocarpum (whole plant) Linn. against Isoniazid & Rifampicin induced hepatic toxicity in wistar rats Pritt Verma 1,2 , Shravan Paswan 1,2 , Surya Prakash Singh 1 , Sajal Shrivastva 2 and Ch.V.Rao 1 1 Pharmacognosy and Ethnopharmacology Division, CSIR-National Botanical Research Institute, Lucknow, India. 2 Amity Institute of Pharmacy, Amity University, Lucknow, India. Tele: E-mail addresses: chvrao72@yahoo.com © 2015 Elixir All rights reserved