Standard practices for Fmoc-based solid-phase
peptide synthesis in the Nowick laboratory
(Version 1.6.1)
Adam G. Kreutzer and Patrick J. Salveson
E-mail:
Contents
Contributions to this guide 3
General 3
Solid Supports 3
Loading 2-chlorotrityl chloride resin .......................... 3
Capping 2-chlorotrityl chloride resin .......................... 4
Loading rink amide resin ................................ 5
Capping Rink amide resin ............................... 6
Check resin loading ................................... 7
Solid-phase peptide synthesis 8
Manual (hand) coupling ................................ 8
Automated synthesis – PS3 synthesizer ........................ 11
Automated synthesis – CEM synthesizer ....................... 12
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