A. König et al. (Eds.): KES 2011, Part III, LNAI 6883, pp. 237–245, 2011. © Springer-Verlag Berlin Heidelberg 2011 Design of Initial Biosensor for Measurement of Glucose in Human Blood by Using Biocomputing Technology Yuyi Chu 1 , Junzo Watada 2 , Ikno Kim 1 , and Juiyu Wu 1 1 Graduate chool of Information, Production and Systems, Waseda University Kitakyushu, Japan 2 Taipei Medical University, Taipei, Taiwan Abstract. Since biocomputing plays an essential role in the biological and medical fields, the catalytic features of enzymes can be utilized in medical applications as biosensor. In this study, we tried to build an enzymatic system as biosensor equipment application to measure blood sugar with sequential reactions of enzymes, which express logic output signals with latent fluorophore as a reporter. The expected biosensor will analyze three molecules sucrose, maltose and ATP existing in human blood, by adding some blood into the system consisted of various enzymes. Sucrose and Maltose will be decomposed into glucose; moreover, glucose and ATP have critical influence of glycolysis which shows one crucial reaction of human metabolism. The output signal was visualized by high throughput fluorescence that originated from the long-wavelength latent fluorogenic substrate—Salicylate hydroxylase (SHL) reaction. The experiment results of this research have showed the possibility to determine the combinations of the three important components, by setting the concentration of each input molecules to specific threshold values. Further researches are required to find out the relationship between the specific concentration and measure glucose results, especially in the medical field. Keywords: Biosensor, Biocomputing, Enzymes, Blood Glucose, Forecast. 1 Introductions Diabetes is a metabolic disease in which a person cannot produce enough insulin or not response to the insulin induce multiple endanger syndrome. In this century, prevention and therapy methods of diabetes have become a main issue of research as well as several effective treatments have held in various aspects. However, there is no radical cure for diabetes that transfers it into chronic disease that pushes medical investigation into improving living quality of patients. Not only monitoring blood sugar but also predicting its variation has ripen into the major subject influencing appropriate decisions regarding treatment plan. Biochemical computing is a branch of unconventional computing research that aims to perform various computations by using biomolecules to mimic electronic computing devices. Conventional computers and other electronic digital devices are based on