© Asian Journal of Biomedical and Pharmaceutical Sciences, 2015. Five novel spectrophotometric methods for quantitative determination of Prulifloxacin in pure and pharmaceutical formulations he main objective of this present work is to successfully develop one UV and four visible new spec- trophotometric techniques. One UV and four visible spectrophotometric methods have been devel- oped depending on the reactivity of various structural units for instance piperazine ring and tertiary amine and carbonyl group in Pruliloxacin. It is observed that the statistical analysis results of cor- relation coeicient of each method values are observed to be greater than 0.999 which speaks that the proposed methods have good linearity. he order of sensitivity of the said proposed methods are M1> M2>M5c> M5b> M5a> M4> M3. he % RSD values are observed bellow two for all methods which shows that all the proposed methods are precise. he results of analysis of the pharmaceutical formulations assert that the proposed methods are safely be adopted for routine quality control of Pruliloxacin in bulk and pharmaceutical preparations to yield correct and accurate analysis re- sults. Keywords: Pruliloxacin, Visible spectrophotometric methods, Bratton-Marshal reagent, Validation. ABSTRACT : Pruliloxacin (PRFX) pertains to prodrug of uliloxacin which is broad spectrum luoroquinolone an anti- bacterial agent. Pruliloxacin is metabolized in the body to the active compound in Uliloxacin. Pruliloxacin is a prodrug and found to be efective on par with Ciproloxacin, co- amoxiclav or Peloloxacin in the treatment of bronchitis exacerbations and lower urinary tract infections. PRFX is not oicial drug in IP, BP and USP pharmacopoeia. In this connection relevant nemerous literature survey of PRFX was compiled and thoroughly examined before development of these methods to have adequate knowledge in this regard. Literature survey disclosed that very few spectrophotometric methods have been reported so far. Majority of HPLC methods were applied in determination of FQs in human plasma 1-5 , edible animal products, feeds and to a lesser extent in pharmaceutical formulations 6-10 . Capillary Zone Electrophoresis 11 methods are in existence to determine the active metabolite of Pruliloxacin existed in human plasma and some other biological luids. It was also reported that there was a sensitive determination of Pruliloxacin owing to its luorescence enhancement on Terbium (III)-Sodium Dodecylbenzene Sulfonate system 12 . here are no analytical reports available to estimate PRFX for utilizing visible spectrophotometry. herefore the author inclined to select PRFX for the development of sensitive, precise and accurate spectrophotometric methods validated according to ICH Q2(R1) guidelines 13 , depending on several chemical reactions duly involving the analytically prominent functional groups existed in the structure. hus the author chooses one ultraviolet and four visible spectrophotometric methods namely M 1 , M 2 , M 3 , M 4 , and M 5a , M 5b , M 5c for determination of PRFX in bulk samples and pharmaceutical formulations. he broad details of diferent spectrophotometric methods developed are shown in Table 1. *Corresponding author: P. RAVISANKAR, E-mail: banuman35@gmail.com Mobile: +919000199106, +919059994000. doi: 10.15272/ajbps.v5i48.724 INTRODUCTION: Received on:11/07/2015 Accepted on: 25/08/2015 Published on: 15/09/2015 QR Code for mobile Article Info: O pen A ccess Literati Research Article Panchumarthy Ravisankar 1 * 1 Department of Pharmaceutical Analysis and Quality Assurance, Vignan Pharmacy College, Vadlamudi, Guntur (Dist.) - 522213, Andhra Pradesh, India. 1 Faculty of Science, Sri Chandrasekharendra Saraswathi Viswa Maha Vidyalaya (SCSVMV University), Enathur, Kanchipuram – 631561, T.N., India. Conlict of interest: Authors reported none submit your manuscript | www.jbiopharm.com