A NEW ALLOCREADIID (TREMATODA) SPECIES FROM FRESHWATER FISH HETERANDRIA BIMACULATA (TELEOSTEI: POECILIIDAE) IN SOUTHEASTERN MEXICO Guillermo Salgado-Maldonado, Juan Manuel Caspeta-Mandujano*, and Gabriela Va ´ zquezÀ Instituto de Biologı ´a, Universidad Nacional Auto ´noma de Me ´ xico, A.P. 70-153, 04510 Me ´ xico D.F., Mexico. e-mail: gsalgado@ibiologia.unam.mx ABSTRACT: Paracreptotrema heterandriae n. sp. (Trematoda: Allocreadiidae) ; is described from the intestine of the freshwater fish Heterandria bimaculata (Teleostei: Poeciliidae) from the upper basin of Rı ´o La Antigua, in Veracruz state, Mexico. < The new species is distinguished from the 3 others in the Paracreptotrema Choudhury, Pe ´rez-Ponce de Leo ´ n, Brooks, and Daverdin, 2006, mainly by having a feeble membranous cirrus sac containing an uncoiled seminal vesicle, instead of a well-developed muscular cirrus sac that encloses coiled seminal vesicle, pars prostatica, and ejaculatory duct as in the previously 3 nominal species. Moreover, eggs of the new species are larger than all others ([measurements in micrometers] eggs of P. heterandriae n. sp. 72.5 [70–75] 3 40 [35–41]; P. blancoi 55.4 [52.5–62.5] 3 38.5 [32.5–42.5]; P. mendezi 46 3 37; P. profundulusi 57 [52–60] 3 27.8 [25–30]). Choudhury et al. (2006) described Paracreptotrema and placed it in the Allocreadiidae. Positioning this genus in that family was somewhat tentative due to the lack of life-cycle information pertaining to it. As stated by Curran et al. (2006), all known cercariae forms for true allocreadiids have eyespots; consequently, their adult forms present diffuse spots in their forebodies. In the course of our studies of freshwater fish helminths of Mexico, we have collected trematodes from the poeciliid Heterandria bimacu- lata (Heckel) that can be clearly identified as belonging to a new species of Paracreptotrema. These specimens have eyespots, or remnants of eyespots, scattered in dorsal forebody. The new species is described in the present study. MATERIALS AND METHODS The reported helminth specimens were collected from H. bimaculata examined between August 2005 and November 2009, taken from several locations at the upper Rı ´o La Antigua basin, on the eastern slope of the Cofre de Perote volcano and the northern slope of the Pico de Orizaba volcano, near Xalapa, Veracruz, Mexico. Localities were a pond at Agua Bendita (19u249410N, 97u009520W; altitude, 1,277 m; n 5 24 fish examined); a creek at Apazapa ´n (19u19932.20N, 96u43933.50W; altitude, 311 m; n 5 49); Rı ´o Tilapa, not positioned; n 5 2); a creek at Rancho Tizapa ´n (19u249240N, 97u009520W; altitude, 1,277 m; n 5 15); a creek at San Miguel (Avestruces) (19u249110N, 97u029310W; altitude, 1,470 m; n 5 9); and a creek at Cocaxtla (19u239430N, 97u029330W; altitude, 1,374 m; n 5 2). = Fish were caught by electrofishing, transported live to the laboratory, and necropsied immediately after capture. Trematodes were fixed in hot 4% formalin. Some specimens were mounted under slight coverslip pressure. Trematodes were stained with Mayer’s paracarmine or Ehrlich’s hematoxylin and mounted whole in Canada balsam. Light microscopy studies were made of 2 paratype Paracreptotrema blancoi Choudhury, Pe ´rez-Ponce de Leo ´ n, Brooks and Daverdin, 2006, specimens borrowed from the Coleccio ´ n Nacional de Helmintos (CNHE), Instituto de Biologı ´a, Universidad Nacional Auto ´ noma de Me ´xico (UNAM), CNHE 5315. Measurements were made only from specimens fixed without pressure and are given in micrometers as the mean followed by the range in parentheses. Drawings were made with the aid of a camera lucida. Holotype and paratype specimens of the new species were deposited in the CNHE, Instituto de Biologı ´a, UNAM. Additional voucher specimens were deposited in the U.S. National Parasite Collection (USNPC), Beltsville, Maryland, and in the Natural History Museum, London, U.K. (NHMUK). DESCRIPTION Paracreptotrema heterandriae n. sp. (Figs. 1–3) General (20 specimens measured): Body 783 (625–1,050) long, ovoid, maximum width 252 (175–375) at level of ventral sucker, at middle of body length, tapering posteriorly. Forebody 402 (300–500) long, hindbody 415 (325–550) long. Two eyespots clearly visible; remnants of eyespot pigment scattered in dorsal forebody. Oral sucker 121 (100–160) long, 113 (87–150) wide, opening subterminal, anteroventrally directed. Several papillae along outer edge of oral sucker visible on all specimens. Ventral sucker protuberant 155 (112–195) long, 163 (117–217) wide. Sucker length ratio, 1:1.3 (0.9–1.6); sucker width ratio, 1:1.4 (1.2–1.7). Prepharynx absent. Pharynx rounded, small, 54 (45–67) long, 59 (50–75) wide. Esophagus short, 57 (22–85). Cecal bifurcation medial between oral sucker and ventral sucker. Caeca extending just anterior to testes, terminating far from posterior end of body. Testes 2, symmetrical to oblique, ellipsoidal in hindbody, but well separated from posterior end of body; left testis 119 (87–150) long, 75 (52–125) wide; right testis 116 (87–150) long, 79 (57–112) wide. Cirrus sac distinct, placed medially along longitudinal body axis. Cirrus sac elongate saccular to subcylindrical, overlapped by the ventral sucker, extending dorsally slightly beyond anterior margin of ventral sucker; cirrus sac membranous, encloses tubular, not-coiled seminal vesicle only as a weak membrane, 114 (100–145) long, 28 (20–35) wide. Pars prostatica weakly developed, tubular, prostatic cells small, rounded. Ejaculatory duct dorsoventrally curved. Cirrus short, unarmed. Genital pore median, just anterior to anterior border of ventral sucker, placed closely to cecal bifurcation. Ovary 76 (55–112) long, 72 (42–112) wide, round or pyriform, entire, dextral or sinistral, situated just posterior to the margin of ventral sucker. Seminal receptacle rounded, situated immedi- ately posterior to ovary. Laurer’s canal not observed. Vitellarium follicular, voluminous and dense; follicles in 2 mostly ventrolateral fields, entering dorsal area of body in small quantities, extending from level of cecal bifurcation to hindbody, overlapping testes and reaching just to posterior margin of testes, not invading postesticular area. Vitelline reservoir dorsal, median, overlapping with Mehlis’ gland. Mehlis’ gland situated medially just posterior to ventral sucker. Uterus extends into area between testes, often filling postesticular area, sometimes overlapping testis. Metraterm occurs ventral relative to cirrus sac, enters ventral aspect to the genital atrium. Eggs few, operculate, yellowish, 72.5 (70–75) long, 40 (35–41) wide (n 5 12). Excretory vesicle I-shaped, tubular, visible in posttesticular region. Excretory pore terminal. Taxonomic summary Type host: Heterandria bimaculata (Heckel), Guatopote, twospot livebearer (Cyprinodontiformes: Poeciliidae). Infection site: Intestine. Type locality: Pond at Agua Bendita (19u249410N, 97u009520W; altitude, 1,277 m), upper basin of the Rı ´o La Antigua, Xalapa, Veracruz, Mexico. Other localities: Creek at Apazapa ´n (19u19932.20N; 96u43933.50W, altitude, 311 m); Rı ´o Tilapa (not positioned); creek at Rancho Tizapa ´n The Journal of Parasitology para-98-02-26.3d 18/1/12 17:35:05 1 Cust # GE-2907 Received 30 June 2011; revised 24 October 2011; accepted 7 November 2011. * Laboratorio de Parasitologı ´a de Animales Silvestres, Facultad de Ciencias Biolo ´ gicas y Centro de Investigaciones Biolo ´ gicas, Universidad Auto ´ noma del Estado de Morelos, Avenida Universidad Nu ´ mero 1001, Colonia Chamilpa, C.P. 62209, Cuernavaca, Morelos, Mexico. { Departamento de Ecologı ´a Vegetal, Instituto de Ecologı ´a, A. C., Km. 2.5 Carretera Antigua a Coatepec No. 351, Congregacio ´ n El Haya, C.P. 91070, Xalapa, Veracruz, Mexico. DOI: 10.1645/GE-2907.1 J. Parasitol., 98(2), 2012, pp. 000–000 F American Society of Parasitologists 2012 0