Share Welcome Sign in | Register | Mobile Journals Home APS Home IS-MPMI Home My Profile Subscribe Search Advanced Search Help About the cover for October 2007 ISSN: 0191-2917 SEARCH MPMI Phytobiomes Phytopathology Plant Disease Advanced Search Resources Subscribe About Plant Disease First Look Most Downloaded Articles Submit a Manuscript Customer Care About My Password Rights and Permissions Plagiarism and Ethics Advertise Open Access ORCID Registry Editor-in-Chief: Alison E. Robertson Published by The American Phytopathological Society Home > Plant Disease > Table of Contents > Abstract Previous Article | Next Article October 2007, Volume 91, Number 10 Page 1365 https://doi.org/10.1094/PDIS-91-10-1365A Disease Notes First Report of Pantoea agglomerans Causing Leaf Blight and Vascular Wilt in Maize and Sorghum in Mexico G. Morales-Valenzuela † , H. V. Silva-Rojas † , and D. Ochoa-Martínez † , Colegio de Postgraduados, Montecillo, Edo. de México, 56230, México ; E. Valadez-Moctezuma † , B. Alarcón-Zúñiga † , and L. X. Zelaya-Molina † , Universidad Autónoma Chapingo, Texcoco, Edo. de México, 56230, México; L. Córdova-Téllez † , L. Mendoza-Onofre † , H. Vaquera- Huerta † , and A. Carballo-Carballo † , Colegio de Postgraduados, Montecillo, Edo. de México, 56230, México; A. Farfán-Gómez † , Universidad Autónoma Metropolitana- Xochimilco, D.F., 04960, México; and G. Ávila-Quezada , † Centro de Investigación en Alimentación y Desarrollo-Delicias, Chihuahua, 33089, México Open Access. Zea mays and Sorghum bicolor are important crops for animal and human nutrition worldwide. In the Central Highland Valley of Mexico, both crops are extremely important, and research is aimed toward increasing yield, disease resistance, and crop adaptation from 1,900- to 2,700-m elevation. In a 3-year field breeding experiment (2004 to 2006), leaf blight and vascular wilt symptoms were frequently observed in contiguous plots of maize and sorghum crops in Montecillo, Mexico and maize plots in Tecamac, Mexico. To identify and characterize the causal agent of these symptoms, isolations were conducted on leaves from areas where healthy and diseased tissues converged. Leaf sections of 1 cm2 from both crops were disinfested, placed on casamino acid-peptone-glucose (CPG) medium, and incubated at 28°C. After 48 h, only yel low colonies were observed and 12 isolates were selected for further characterization. Physiological and biochemical tests indicated that the isolates were nonfluorescent on King's B medium, and API 50 CHE (bioMérieux, Marcy l'Etoile, France) revealed that they were negative for gelatin hydrolysis, indole production, acid production from raffinose and positive for utilization of glycerol, D-glucose, mannitol, arbutine, esculine, salicine, cellobiose, maltose, melibiose, D-fucose, and D-arabitol; all characteristics of Pantoea agglomerans. Further identification of these isolates was accomplished by DNA analysis. For DNA analysis, 1.4-kbp fragments of the 16S rRNA gene were amplified with primer set 8F/1492R (3) and sequenced with U514F/800R universal primers (2). Five sequences were obtained and deposited in GenBank (Accession Nos. EF050806 to EF050810). A phylogenetic tree was constructed using the UPGMA method (mega version 3.1). Results of the phylogenetic analysis grouped the species P. ananatis, P. stewartti , and P. agglomerans into three clusters. The five unknown sequences were grouped into the P. agglomerans cluster. There was a 98 to 99% Quick Links Add to favorites E-mail to a colleague Alert me when new articles cite this article Download to citation manager Related articles found in APS Journals Books Home First Report of Pantoea agglomerans Causing Leaf Blight and Vascula... http://apsjournals.apsnet.org/doi/abs/10.1094/PDIS-91-10-1365A 1 de 3 22/06/2017 09:42 a. m.