Singh et al., IJPSR, 2017; Vol. 8(3): 1420-1426. E-ISSN: 0975-8232; P-ISSN: 2320-5148 International Journal of Pharmaceutical Sciences and Research 1420 IJPSR (2017), Vol. 8, Issue 3 (Research Article) Received on 06 September, 2016; received in revised form, 14 October, 2016; accepted, 22 October, 2016; published 01 March, 2017 ASSESSMENT OF BIOCHEMICAL PARAMETERS IN THE PATIENTS OF CORONARY ARTERY DISEASE WITH TYPE 2 DIABETES MELLITUS Manisha Singh 1 , Eqbal Anwer 2 and Vishnu Kumar *1 Department of Biochemistry 1 , Department of Physiology 2 , Era’s Lucknow Medical College & Hospital, Sarfaraz Ganj, Hardoi Road, Lucknow - 226003, Uttar Pradesh, India. ABSTRACT: Impaired carbohydrate and lipid metabolism with amplified oxidative stress are well recognized factors for Coronary artery disease (CAD) which is the major reason of mortality and morbidity all over world. The incident of CAD with type 2 diabetes mellitus (T2DM) is growing and they are predicted to be the biggest cause of death by 2020 in India. Therefore, the venture of the present study was to assess the association of hyperglycemia, oxidative stress, antioxidant enzymes, lipid profile and lipoprotein components in serum of CAD with T2DM patients as well as to compare the results with age-sex matched healthy human volunteers. Five hundred participants were enrolled for the present study, with their ages ranging from 37 to 57 years from OPD of medicine, Era’s Lucknow Medical College & Hospital, Lucknow, were attending OPD for routine medical checkup. Out of which two hundred fifty were clinically new diagnosed case of CAD with T2DM like hyperglycemia, angina pectoris and Myocardial infarction (MI), remaining two hundred fifty were healthy controls. All biochemical assays were carried out by the standard kit methods. Participants were investigated for serum lipid profile, Lipoprotein constituents along with levels of lipid peroxide, reduced glutathione and antioxidant enzymes. A marked impairment in levels of lipid profile accompanied with increase in the lipids and apo-protein levels of serum β lipoproteins following decrease in lipid and protein constituents of α lipoprotein, serum reduced glutathione as well as level of antioxidant enzymes in CAD with T2DM patients in comparison to healthy human controls. INTRODUCTION: Dyslipipoproteinemia is an independent risk factor for the development of coronary artery diseases, myocardial infarction, and hypertension in hyperlipidemic patients 1 . Clinically diabetic patients are characterized by marked increase in blood glucose level followed by normal or mild hyperlipidemia. QUICK RESPONSE CODE DOI: 10.13040/IJPSR.0975-8232.8(3).1420-26 Article can be accessed online on: www.ijpsr.com DOI link: http://dx.doi.org/10.13040/IJPSR.0975-8232.8 (3).1420-26 Elevated level in low density lipoprotein (LDL) along with triglyceride especially in very low density lipoprotein (VLDL) and cholesterol in low density lipoprotein with free radicals mediated formation of modified LDL is recognized as a leading cause of development of Atherosclerosis in CAD with T2DM patients 2 . Furthermore hyperlipidemia may also induce abnormalities like resistance to insulin in muscle and liver cells in CAD with T2DM patients. Treatment of hyperlipidemia with available lipid lowering drugs: fibrats and bile acid sequestrants are not free from many side effects such as Keywords: Carbohydrate, Lipid metabolism, Antioxidant, enzymes Correspondence to Author: Dr. Vishnu Kumar Associate Professor, Department of Biochemistry, Era’s Lucknow Medical College & Hospital, Sarfaraz Ganj, Hardoi Road, Lucknow-226003, U.P., India. E-mail: vkawasthi@hotmail.com