Molecular and microscopic analysis of the gut contents of abundant rove beetle species... 1 Molecular and microscopic analysis of the gut contents of abundant rove beetle species (Coleoptera, Staphylinidae) in the boreal balsam fir forest of Quebec, Canada Jan Klimaszewski 1 , Marie-Josee Morency 1 , Philippe Labrie 1 , Armand Séguin 1 , David Langor 2 , Timothy Work 3 , Caroline Bourdon 1 , Evelyne hifault 1 , David Paré 1 , Alfred F. Newton 4 , Margaret K. hayer 4 1 Natural Resources Canada, Canadian Forest Service, Laurentian Forestry Centre, 1055 du P.E.P.S., P.O. Box 10380, Stn. Sainte-Foy, Québec, Québec, G1V 4C7, Canada 2 Natural Resources Canada, Canadian Forest Service, Northern Forestry Centre, 5320-122 Street, Edmonton, Alberta, T6H 3S5, Canada 3 Départe- ment des sciences biologiques, Université du Québec à Montréal, CP 8888, Succursale Centre-ville, Montréal, Québec, H3C 3P8, Canada 4 Integrative Research Center, he Field Museum of Natural History, Chicago, Illinois, 60605-2496, U.S.A. Corresponding author: Jan Klimaszewski (jan.klimaszewski@nrcan-rncan.gc.ca) Academic editor: Michael Ivie  |  Received 18 July 2013  |  Accepted 15 October 2013  |  Published 20 November 2013 Citation: Klimaszewski J, Morency M-J, Labrie P, Séguin A, Langor D, Work T, Bourdon C, hifault E, Paré D, Newton AF, hayer MK (2013) Molecular and microscopic analysis of the gut contents of abundant rove beetle species (Coleoptera, Staphylinidae) in the boreal balsam ir forest of Quebec, Canada. ZooKeys 353: 1–24. doi: 10.3897/ zookeys.353.5991 Abstract Experimental research on beetle responses to removal of logging residues following clearcut harvesting in the boreal balsam ir forest of Quebec revealed several abundant rove beetle (Staphylinidae) species potentially important for long-term monitoring. To understand the trophic ailiations of these species in forest ecosys- tems, it was necessary to analyze their gut contents. We used microscopic and molecular (DNA) methods to identify the gut contents of the following rove beetles: Atheta capsularis Klimaszewski, Atheta klagesi Bern- hauer, Oxypoda grandipennis (Casey), Bryophacis smetanai Campbell, Ischnosoma longicorne (Mäklin), Myce- toporus montanus Luze, Tachinus frigidus Erichson, Tachinus fumipennis (Say), Tachinus quebecensis Robert, and Pseudopsis subulata Herman. We found no apparent arthropod fragments within the guts; however, a number of fungi were identiied by DNA sequences, including ilamentous fungi and budding yeasts [As- comycota: Candida derodonti Suh & Blackwell (accession number FJ623605), Candida mesenterica (Geiger) Diddens & Lodder (accession number FM178362), Candida railenensis Ramirez and Gonzáles (accession number JX455763), Candida sophie-reginae Ramirez & González (accession number HQ652073), Candida ZooKeys 353: 1–24 (2013) doi: 10.3897/zookeys.353.5991 www.zookeys.org Copyright Her Majesty the Queen in Right of Canada. This is an open access article distributed under the terms of the Creative Commons Attribution Li- cense 3.0 (CC-BY), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. RESEARCH ARTICLE Launched to accelerate biodiversity research A peer-reviewed open-access journal