International Journal of Public Health Science (IJPHS) Vol. 1, No. 1, July 2012, pp. 7~10 ISSN: 2252-8806  7 Journal homepage: http://iaesjournal.com/online/index.php/IJPHS Study of Oxidative Stress in Relation with Antioxidant Status in Chronic Bronchitis Anita M Raut*, A.N. Suryakar**, Dilip Mhaisekar*** *Dr. Vikhe Patil Institute of Medical Sciences, Ahmednagar **Prof. & Registrar MUHS, Nashik ***Dr. Shankarrao Chavan Govt. Medical College, Nanded Article Info ABSTRACT Article history: Received May 16, 2012 Revised May 28, 2012 Accepted June 4, 2012 Lipid peroxide plays an important role in inflammatory lung diseases. Increased epithelial permeability produced by cigarette smoke is likely to be mediated though depletion of the Total Antioxidant Capacity .Oxidative stress has been recognized as a central feature of smoke induced chronic bronchitis. Imbalance between oxidants and Total Anioxidant Capacity is also an established fact in these patients. 60 patients with chronic bronchitis included in the study. Their base line clinical examination, malondialdehyde (MDA), nitric oxide, alpha tochopherol and Total Antioxidant Capacity were measured. 100 healthy non-smokers’ were served as controls. The mean malondialdehyde levels and nitric oxide in the patients at base line were higher than Controls (p<0.001). Plasma alpha-tocopherol and total antioxidant capacity were lower (p<0.001) in the patients compared to controls. The present study shows that initially the plasma lipid peroxide (MDA) levels were high and antioxidants. (alpha- tocopherol, total antioxidant capacity) were low in patients with chronic bronchitis. Our results suggest the presence of oxidative stress and decrease in total antioxidant capacity in chronic bronchitis. Keyword: Alpha-tocopherol Chronic bronchitis Malondialdehyde Total antioxidant capacity Copyright © 2012 Institute of Advanced Engineering and Science. All rights reserved. Corresponding Author: Dr. Mrs. Anita M. Raut Dept of Biochemistry Dr. Vikhe Patil Institute of Medical Sciences Ahmednagar (M S), India Email: anitaraut009@gmail.com M-9270019213, 8421753938 1. INTRODUCTION Lung is the organ which is constantly exposed to many atmospheric pollutants such as cigarette smoke, ozone and nitrogen dioxide and is also at risk from oxidant injury by inhalation [1]. Inhaled ozone induces toxic processes that impair lung function. Lipid peroxide plays an important role in inflammatory lung diseases. Increased epithelial permeability produced by cigarette smoke is likely to be mediated though depletion Total Antioxidant Capacity [2]. Oxidant-antioxidant balance is essential for the normal lung function. Both an increased oxidants and or decreased antioxidants may reverse the physiologic oxidant- antioxidants balance in favors of oxidants leading to lung injury. Chronic bronchitis is defined clinically that it is present in any patient who has persistant cough with sputum production for at least two consecutive years, in the absence of any other identifiable cause. In simple chronic bronchitis, patients have a productive cough but no physiologic evidence of airflow obstruction. Where some individual may demonstrate hyperreactive airways with intermittent bronchospasm and wheezing. This condition is called chronic asthmatic bronchitis some patients, especially heavy smokers,