Title: Patient-Derived GM-CSF Autoantibodies Reproduce Pulmonary Alveolar Proteinosis in Non-Human Primates Running head: GM-CSF Autoantibodies Reproduce PAP Authors: Takuro Sakagami, 1 David Beck, 1,2 Kanji Uchida, 1 Takuji Suzuki, 1 Brenna C. Carey, 1 Koh Nakata, 3 Gary Keller, 4 Robert E. Wood, 5 Susan E. Wert, 1 Machiko Ikegami, 1 Jeffrey A. Whitsett, 1 Maurizio Luisetti, 6 Stella Davies, 7 Jeffrey P. Krischer, 8 Alan Brody, 9 Fred Ryckman, 10 and Bruce C. Trapnell. 1,2,5 Institutions: Divisions of 1 Pulmonary Biology, 4 Veterinarian Services; 5 Pulmonary Medicine, 7 Experimental Hematology, 9 Radiology and 10 Transplant Surgery, Cincinnati Children’s Hospital Medical Center; 2 Division of Critical Care, Pulmonary and Sleep Medicine, University of Cincinnati - both in Cincinnati, OH, 45229-3039, USA; 3 Bioscience Medical Research Center, Niigata Medical and Dental Hospital, Niigata, Japan; Institute for Respiratory Disease, 6 San Matteo Hospital Foundation for Research and Care, University of Pavia, Pavia, Italy; 8 Division of Informatics and Biostatistics, University of South Florida, Tampa, FL, USA. Source of support: National Heart Lung and Blood Institute (HL0085453 to BCT and HL085610 to JAW), National Center for Research Resources and the National Institutes of Health Office of Rare Diseases (RR019498 to BCT). Subject: Diffuse lung diseases Key words: Alveolar macrophages, Surfactant homeostasis, Autoimmunity, Neutrophils, Inflammation, Anti-GM-CSF Therapy Correspondence: Bruce C. Trapnell, MD Division of Pulmonary Biology Cincinnati Children’s Hospital Medical Center 3333 Burnet Avenue Cincinnati, OH 45229-3039 Phone: 513.636.6361 Fax: 513.636.3723 Email: Bruce.Trapnell@cchmc.org Word count: 4634 This article has an online data supplement which is available from this issue’s table of content online at www.atsjournals.org Page 1 of 69 Media embargo until 2 weeks after above posting date; see thoracic.org/go/embargo AJRCCM Articles in Press. Published on March 11, 2010 as doi:10.1164/rccm.201001-0008OC Copyright (C) 2010 by the American Thoracic Society.