© 2017. Published by The Company of Biologists Ltd. Unique Cell Biological Profiles of Retinal Disease-Causing Missense Mutations in the Polarity Protein Crumbs Milena Pellikka and Ulrich Tepass Department of Cell and Systems Biology, University of Toronto, Toronto, Ontario, Canada Key words: Crumbs, CRB1, photoreceptor cells, retinal degeneration, missense mutations Corresponding author: Ulrich Tepass Department of Cell and Systems Biology University of Toronto 25 Harbord Street Toronto, Ontario M5S 3G5 Canada Phone: 416-978-5712 Email: u.tepass@utoronto.ca Summary Statement To better understand the consequences of retinal disease causing missense mutations in human CRB1 we have recreated 4 mutations in Drosophila crb. Analysis of these mutations identifies domains within the Crb extracellular region that control protein level and distribution, and suggests that CRB1 missense mutations affect rhodopsin trafficking, a known cause of retinal disease. Journal of Cell Science  Advance article JCS Advance Online Article. Posted on 17 May 2017